7P7Y

X-ray structure of Lactobacillus kefir alcohol dehydrogenase mutant Q126K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	NADPH dependent R-specific alcohol dehydrogenase	polymer	260	27711.3	1	UniProt (Q6WVP7) Pfam (PF13561) In PDB	Lactobacillus kefiri	NADPH dependent R-specific alcohol dehydrogenase,SDR family NAD(P)-dependent oxidoreductase
2	A	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
3	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5		water	water		18.0	344	Chemie (HOH)

Sequence modifications

A: 1 - 251 (UniProt: Q6WVP7)

PDB	External Database	Details
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Lys 126	Gln 127	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27711.3
Non-Polymers*	Number of molecules	6
	Total formula weight	243.7
All*	Total formula weight	27954.9

*Water molecules are not included.