7P39

4,6-alpha-glucanotransferase GtfB from Limosilactobacillus reuteri NCC 2613 complexed with acarbose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Dextransucrase	polymer	881	97834.0	2	UniProt (A0A1Z2RUH3)	Lactobacillus reuteri	Sucrose 6-glucosyltransferase
2	C, D (D, G)	4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		645.6	2	In PDB BIRD (PRD_900022) GlyTouCan (G08743RW)
3	E, G (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	F (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	H, I (A, B)	water	water		18.0	19	Chemie (HOH)

Sequence modifications

A, B: 417 - 1277 (UniProt: A0A1Z2RUH3)

PDB	External Database	Details
Met 397	-	initiating methionine
Ala 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
Ser 405	-	expression tag
Ala 406	-	expression tag
Ala 407	-	expression tag
Leu 408	-	expression tag
Glu 409	-	expression tag
Val 410	-	expression tag
Leu 411	-	expression tag
Phe 412	-	expression tag
Gln 413	-	expression tag
Gly 414	-	expression tag
Pro 415	-	expression tag
Gly 416	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	195668.1
Branched	Number of molecules	2
	Total formula weight	1291.2
Non-Polymers*	Number of molecules	3
	Total formula weight	176.2
All*	Total formula weight	197135.5

*Water molecules are not included.