7P30

3.0 A resolution structure of a DNA-loaded MCM double hexamer

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, G (2, A)	DNA replication licensing factor MCM2	polymer	868	98911.5	2	UniProt (P29469) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Minichromosome maintenance protein 2
2	B, H (3, B)	DNA replication licensing factor MCM3	polymer	1006	111720.2	2	UniProt (P24279) Pfam (PF22207) Pfam (PF23191) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Minichromosome maintenance protein 3
3	C, I (4, C)	DNA replication licensing factor MCM4	polymer	933	105138.4	2	UniProt (P30665) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cell division control protein 54
4	D, J (5, D)	Minichromosome maintenance protein 5	polymer	775	86505.7	2	UniProt (P29496) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cell division control protein 46
5	E, K (6, E)	DNA replication licensing factor MCM6	polymer	1017	113110.2	2	UniProt (P53091) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Minichromosome maintenance protein 6
6	F, L (7, F)	DNA replication licensing factor MCM7	polymer	845	95049.9	2	UniProt (P38132) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF24901)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Cell division control protein 47,Minichromosome maintenance protein 7
7	M (X)	DNA (53-MER)	polymer	53	16326.4	1		Saccharomyces cerevisiae S288C
8	N (Y)	DNA (53-MER)	polymer	53	16335.5	1		Saccharomyces cerevisiae S288C
9	DA, O (A, 2)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
10	BA, EA, HA, LA, OA... (7, A, B, D, F...)	MAGNESIUM ION	non-polymer		24.3	10	Chemie (MG)
11	CA, FA, JA, MA, NA... (7, A, C, D, E...)	ZINC ION	non-polymer		65.4	10	Chemie (ZN)
12	AA, GA, IA, KA, PA... (7, B, C, D, F...)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	8	Chemie (ADP)

Sequence modifications

3, B: 1 - 971 (UniProt: P24279)

PDB	External Database	Details
Met -34	-	initiating methionine
Lys -33	-	expression tag
Arg -32	-	expression tag
Arg -31	-	expression tag
Trp -30	-	expression tag
Lys -29	-	expression tag
Lys -28	-	expression tag
Asn -27	-	expression tag
Phe -26	-	expression tag
Ile -25	-	expression tag
Ala -24	-	expression tag
Val -23	-	expression tag
Ser -22	-	expression tag
Ala -21	-	expression tag
Ala -20	-	expression tag
Asn -19	-	expression tag
Arg -18	-	expression tag
Phe -17	-	expression tag
Lys -16	-	expression tag
Lys -15	-	expression tag
Ile -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Glu 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	1253533.9
Non-Polymers*	Number of molecules	30
	Total formula weight	5329.1
All*	Total formula weight	1258863.0

*Water molecules are not included.