7P1Y

A small alarmone hydrolase TdActApo2 mutant - T78N

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (BBB, DDD, CCC, AAA)	HD domain-containing protein	polymer	232	26581.6	4	UniProt (Q73M21)	Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104)
2	E, F, H, J (BBB, DDD, CCC, AAA)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN) PubChem (27854)
3	G, I (CCC, AAA)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL) PubChem (312)
4	K, L, M, N (BBB, DDD, CCC, AAA)	water	water		18.0	87	Chemie (HOH)

Sequence modifications

BBB, DDD, CCC, AAA: 1 - 205 (UniProt: Q73M21)

PDB	External Database	Details
Met -26	-	initiating methionine
Gly -25	-	expression tag
Ser -24	-	expression tag
Ser -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Glu -14	-	expression tag
Asn -13	-	expression tag
Leu -12	-	expression tag
Tyr -11	-	expression tag
Phe -10	-	expression tag
Gln -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asn 78	Asp 78	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	106326.5
Non-Polymers*	Number of molecules	6
	Total formula weight	290.7
All*	Total formula weight	106617.1

*Water molecules are not included.