7P1H

Structure of the V. vulnificus ExoY-G-actin-profilin complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Maltose/maltodextrin-binding periplasmic protein,RTX-toxin	polymer	827	91467.5	1	UniProt (P0AEX9) UniProt (A0A0F6NGV0) Pfam (PF01547) UniProt (by SIFTS) (A0A023NA98)	Escherichia coli (strain K12)	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
2	B (B)	Actin, cytoplasmic 1	polymer	372	41402.2	1	UniProt (P60709) Pfam (PF00022)	Homo sapiens (Human)	Beta-actin
3	C (P)	Profilin-1	polymer	140	15071.2	1	UniProt (P07737) Pfam (PF00235)	Homo sapiens (Human)	Epididymis tissue protein Li 184a,Profilin I
4	D (B)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
5	E (B)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)

Sequence modifications

A: -376 - -11 (UniProt: P0AEX9)

PDB	External Database	Details
Met -397	-	initiating methionine
Gly -396	-	expression tag
Ser -395	-	expression tag
Ser -394	-	expression tag
His -393	-	expression tag
His -392	-	expression tag
His -391	-	expression tag
His -390	-	expression tag
His -389	-	expression tag
His -388	-	expression tag
Ser -387	-	expression tag
Ser -386	-	expression tag
Gly -385	-	expression tag
Leu -384	-	expression tag
Val -383	-	expression tag
Pro -382	-	expression tag
Arg -381	-	expression tag
Gly -380	-	expression tag
Ser -379	-	expression tag
His -378	-	expression tag
Met -377	-	expression tag
Val -65	Ala 338	conflict
Asn -10	-	linker
Ser -9	-	linker
Gly -8	-	linker
Ser -7	-	linker
Ser -6	-	linker
Gly -5	-	linker
Ser -4	-	linker
Ser -3	-	linker
Val -2	-	linker
Glu -1	-	linker

A: 0 - 429 (UniProt: A0A0F6NGV0)
B: 4 - 375 (UniProt: P60709)

PDB	External Database	Details
Ala 272	Cys 272	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	147941.0
Non-Polymers*	Number of molecules	2
	Total formula weight	547.3
All*	Total formula weight	148488.3

*Water molecules are not included.