7OYT

E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D F88L in complex with spermidine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Putrescine-binding periplasmic protein PotF	polymer	352	39496.6	1	UniProt (P31133) Pfam (PF13416) In PDB	Escherichia coli (strain K12)
2	A	SPERMIDINE	non-polymer		145.2	1	Chemie (SPD)
3	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
5	A	1,2-ETHANEDIOL	non-polymer		62.1	17	Chemie (EDO)
6	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
7	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
8	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9		water	water		18.0	211	Chemie (HOH)

Sequence modifications

A: 27 - 370 (UniProt: P31133)

PDB	External Database	Details
Thr 38	Ser 38	engineered mutation
Tyr 87	Ser 87	engineered mutation
Leu 88	Phe 88	engineered mutation
Asp 182	Ala 182	engineered mutation
Ser 247	Asp 247	engineered mutation
Trp 276	Phe 276	engineered mutation
Gln 348	Leu 348	engineered mutation
Leu 371	-	expression tag
Glu 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39496.6
Non-Polymers*	Number of molecules	25
	Total formula weight	1841.5
All*	Total formula weight	41338.1

*Water molecules are not included.