7OYS

E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D Y87S in complex with spermidine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Putrescine-binding periplasmic protein	polymer	352	39454.6	1	UniProt (A0A6S5NLK9) Pfam (PF13416) UniProt (by SIFTS) (P31133)	Escherichia coli
2	B (A)	SPERMIDINE	non-polymer		145.2	1	Chemie (SPD)
3	C, D, E, F, G... (A)	1,2-ETHANEDIOL	non-polymer		62.1	15	Chemie (EDO)
4	I (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	O (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	T, U (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
7	V (A)	water	water		18.0	301	Chemie (HOH)

Sequence modifications

A: 27 - 370 (UniProt: A0A6S5NLK9)

PDB	External Database	Details
Thr 38	Ser 21	engineered mutation
Asp 182	Ala 165	engineered mutation
Ser 247	Asp 230	engineered mutation
Trp 276	Phe 259	engineered mutation
Gln 348	Leu 331	engineered mutation
Leu 371	-	expression tag
Glu 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39454.6
Non-Polymers*	Number of molecules	20
	Total formula weight	1402.0
All*	Total formula weight	40856.6

*Water molecules are not included.