7OYS
E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D Y87S in complex with spermidine
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Putrescine-binding periplasmic protein | polymer | 352 | 39454.6 | 1 | UniProt (A0A6S5NLK9) Pfam (PF13416) UniProt (by SIFTS) (P31133) In PDB | Escherichia coli | |
2 | A | SPERMIDINE | non-polymer | 145.2 | 1 | Chemie (SPD) | |||
3 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 15 | Chemie (EDO) | |||
4 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
5 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
6 | A | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
7 | water | water | 18.0 | 301 | Chemie (HOH) |
Sequence modifications
A: 27 - 370 (UniProt: A0A6S5NLK9)
PDB | External Database | Details |
---|---|---|
Thr 38 | Ser 21 | engineered mutation |
Asp 182 | Ala 165 | engineered mutation |
Ser 247 | Asp 230 | engineered mutation |
Trp 276 | Phe 259 | engineered mutation |
Gln 348 | Leu 331 | engineered mutation |
Leu 371 | - | expression tag |
Glu 372 | - | expression tag |
His 373 | - | expression tag |
His 374 | - | expression tag |
His 375 | - | expression tag |
His 376 | - | expression tag |
His 377 | - | expression tag |
His 378 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 39454.6 | |
Non-Polymers* | Number of molecules | 20 |
Total formula weight | 1402.0 | |
All* | Total formula weight | 40856.6 |