7OXQ

Crystal structure of HIV-1 reverse transcriptase with a double stranded DNA in complex with fragment 048 at the transient P-pocket.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Reverse transcriptase/ribonuclease H	polymer	556	64038.4	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) In PDB	Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1)	Exoribonuclease H,p66 RT
2	B, D	Gag-Pol polyprotein	polymer	444	51928.6	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) In PDB	Human immunodeficiency virus type 1 BH10	Pr160Gag-Pol
3	T, E	DNA (28-MER)	polymer	28	8680.6	2		Human immunodeficiency virus type 1 BH10
4	P, F	DNA (5'-D(*AP*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*GP*CP*GP*CP*CP*G)-3')	polymer	21	6416.1	2		Homo sapiens
5	D, E, F, A, B...	CADMIUM ION	non-polymer		112.4	21	Chemie (CD)
6	C	2-(4-bromanylpyrazol-1-yl)-~{N}-cyclopropyl-~{N}-methyl-ethanamide	non-polymer		258.1	1	Chemie (2NU)

Sequence modifications

A, C: 1 - 554 (UniProt: P03366)

PDB	External Database	Details
Met -1	-	initiating methionine
Val 0	-	expression tag
Cys 63	Ile 662	conflict
Ser 280	Cys 879	conflict

B, D: 1 - 428 (UniProt: P03366)

PDB	External Database	Details
Met -15	-	initiating methionine
Ala -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ala -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ser 280	Cys 879	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	262127.4
Non-Polymers*	Number of molecules	22
	Total formula weight	2618.7
All*	Total formula weight	264746.2

*Water molecules are not included.