7OXH

ttSlyD with pseudo-wild-type S2 peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Peptidyl-prolyl cis-trans isomerase	polymer	158	17400.2	1	UniProt (Q5SLE7) Pfam (PF00254)	Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
2	B (B)	30S ribosomal protein S2	polymer	15	1775.1	1	UniProt (P0A7V0)	Escherichia coli (strain K12)	Small ribosomal subunit protein uS2
3	C (E)	Fragment of 30S ribosomal protein S2 peptide	polymer	2	188.2	1		synthetic construct
4	D, E, G (A)	2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL	non-polymer		354.4	3	Chemie (PE4)
5	F (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
6	H, I (A)	NICKEL (II) ION	non-polymer		58.7	2	Chemie (NI)
7	J (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
8	K, L (A, B)	water	water		18.0	75	Chemie (HOH)

Sequence modifications

A: 1 - 149 (UniProt: Q5SLE7)

PDB	External Database	Details
Pro 150	-	expression tag
Ser 151	-	expression tag
Gly 152	-	expression tag
His 153	-	expression tag
His 154	-	expression tag
His 155	-	expression tag
His 156	-	expression tag
His 157	-	expression tag
His 158	-	expression tag

B: 1 - 15 (UniProt: P0A7V0)

PDB	External Database	Details
Ala 6	Pro 25	engineered mutation
Leu 9	Lys 28	engineered mutation
Ala 12	Ile 31	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	19363.5
Non-Polymers*	Number of molecules	7
	Total formula weight	1322.3
All*	Total formula weight	20685.8

*Water molecules are not included.