7OX7
Target-bound SpCas9 complex with TRAC chimeric RNA-DNA guide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | chimeric RNA-DNA guide | polymer | 81 | 25995.6 | 1 | synthetic construct | ||
| 2 | B (B) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1372 | 158875.0 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes serotype M1 | SpCas9,SpyCas9 |
| 3 | C (C) | TRAC target DNA strand | polymer | 28 | 8574.5 | 1 | synthetic construct | ||
| 4 | D (D) | TRAC non-target DNA strand | polymer | 12 | 3677.4 | 1 | synthetic construct | ||
| 5 | E, F (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | G, H, I, J, K... (A, B) | POTASSIUM ION | non-polymer | 39.1 | 14 | Chemie (K) | |||
| 7 | U, V, W, X (A, B, C, D) | water | water | 18.0 | 384 | Chemie (HOH) |
Sequence modifications
B: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 197122.6 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 596.0 | |
| All* | Total formula weight | 197718.6 |
