7OTQ
Cryo-EM structure of ALC1/CHD1L bound to a PARylated nucleosome
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | K | Chromodomain-helicase-DNA-binding protein 1-like | polymer | 872 | 98906.8 | 1 | UniProt (Q86WJ1) Pfam (PF00176) Pfam (PF00271) In PDB | Homo sapiens (Human) | Amplified in liver cancer protein 1 |
2 | A, E | Histone H3.2 | polymer | 136 | 15403.1 | 2 | UniProt (P84233) Pfam (PF00125) In PDB | Xenopus laevis (African clawed frog) | |
3 | B, F | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62799) Pfam (PF15511) In PDB | Xenopus laevis (African clawed frog) | |
4 | C, G | Histone H2A type 1 | polymer | 130 | 14109.4 | 2 | UniProt (P06897) Pfam (PF00125) Pfam (PF16211) In PDB | Xenopus laevis (African clawed frog) | |
5 | D, H | Histone H2B 1.1 | polymer | 123 | 13655.9 | 2 | UniProt (P02281) Pfam (PF00125) In PDB | Xenopus laevis (African clawed frog) | H2B1.1 |
6 | I | DNA (149-MER) Widom 601 sequence | polymer | 160 | 49175.3 | 1 | synthetic construct | ||
7 | J | DNA (149-MER) Widom 601 sequence | polymer | 160 | 49606.6 | 1 | synthetic construct |
Sequence modifications
K: 16 - 879 (UniProt: Q86WJ1)
A, E: 0 - 135 (UniProt: P84233)
C, G: 0 - 129 (UniProt: P06897)
D, H: 1 - 122 (UniProt: P02281)
PDB | External Database | Details |
---|---|---|
Met 15 | - | initiating methionine |
Ala 880 | - | expression tag |
His 881 | - | expression tag |
His 882 | - | expression tag |
His 883 | - | expression tag |
His 884 | - | expression tag |
His 885 | - | expression tag |
His 886 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ala 102 | Gly 103 | conflict |
Ala 110 | Cys 111 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Arg 99 | Gly 100 | conflict |
Ser 123 | Ala 124 | conflict |
PDB | External Database | Details |
---|---|---|
Met 0 | - | initiating methionine |
Thr 29 | Ser 33 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 11 |
Total formula weight | 306814.4 | |
All* | Total formula weight | 306814.4 |