7OSB
Crystal Structure of a Double Mutant PETase (S238F/W159H) from Ideonella sakaiensis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Poly(ethylene terephthalate) hydrolase | polymer | 298 | 31354.0 | 3 | UniProt (A0A0K8P6T7) Pfam (PF01738) | Ideonella sakaiensis | PET hydrolase,PETase,PET-digesting enzyme |
| 2 | D, F, G, H, I... (A, B, C) | SULFATE ION | non-polymer | 96.1 | 10 | Chemie (SO4) | |||
| 3 | E, K, Q (A, B, C) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 4 | J, P (B, C) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 5 | S, T, U (A, B, C) | water | water | 18.0 | 1082 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 290 (UniProt: A0A0K8P6T7)
| PDB | External Database | Details |
|---|---|---|
| His 159 | Trp 159 | engineered mutation |
| Phe 238 | Ser 238 | engineered mutation |
| Leu 291 | - | linker |
| Glu 292 | - | linker |
| His 293 | - | expression tag |
| His 294 | - | expression tag |
| His 295 | - | expression tag |
| His 296 | - | expression tag |
| His 297 | - | expression tag |
| His 298 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 94061.9 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 1251.2 | |
| All* | Total formula weight | 95313.0 |
