7OSB

Crystal Structure of a Double Mutant PETase (S238F/W159H) from Ideonella sakaiensis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Poly(ethylene terephthalate) hydrolase	polymer	298	31354.0	3	UniProt (A0A0K8P6T7) Pfam (PF01738) In PDB	Ideonella sakaiensis	PET hydrolase,PETase,PET-digesting enzyme
2	A, B, C	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
3	A, B, C	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
4	B, C	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5		water	water		18.0	1082	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 290 (UniProt: A0A0K8P6T7)

PDB	External Database	Details
His 159	Trp 159	engineered mutation
Phe 238	Ser 238	engineered mutation
Leu 291	-	linker
Glu 292	-	linker
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	94061.9
Non-Polymers*	Number of molecules	15
	Total formula weight	1251.2
All*	Total formula weight	95313.0

*Water molecules are not included.