7ORA
Crystal structure of the T478K mutant receptor binding domain of SARS-CoV-2 Spike glycoprotein in complex with COVOX-45 and COVOX-253 Fabs
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | R, C | Spike protein S1 | polymer | 205 | 23179.0 | 2 | UniProt (P0DTC2) Pfam (PF09408) In PDB | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2) | T478K mutant RBD |
2 | A, D | COVOX-45 Fab heavy chain | polymer | 226 | 24187.2 | 2 | Homo sapiens | ||
3 | B, E | COVOX-45 Fab light chain | polymer | 214 | 23359.8 | 2 | Homo sapiens | ||
4 | F, H | COVOX-253 Fab heavy chain | polymer | 228 | 24371.4 | 2 | Homo sapiens | ||
5 | G, L | COVOX-253 Fab light chain | polymer | 215 | 23256.6 | 2 | Homo sapiens | ||
6 | I | alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 367.3 | 1 | In PDB GlyTouCan (G86851RC) | |||
7 | J, K, M | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 570.5 | 3 | In PDB GlyTouCan (G21290RB) | |||
8 | R, F, C, L | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
9 | A, B, F, H | CHLORIDE ION | non-polymer | 35.5 | 6 | Chemie (CL) | |||
10 | water | water | 18.0 | 283 | Chemie (HOH) |
Sequence modifications
R, C: 333 - 528 (UniProt: P0DTC2)
PDB | External Database | Details |
---|---|---|
Glu 324 | - | expression tag |
Thr 325 | - | expression tag |
Gly 326 | - | expression tag |
His 327 | - | expression tag |
His 328 | - | expression tag |
His 329 | - | expression tag |
His 330 | - | expression tag |
His 331 | - | expression tag |
His 332 | - | expression tag |
Lys 478 | Thr 478 | engineered mutation |
Lys 527 | Pro 527 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 10 |
Total formula weight | 236707.9 | |
Branched | Number of molecules | 4 |
Total formula weight | 2079.0 | |
Non-Polymers* | Number of molecules | 12 |
Total formula weight | 765.3 | |
All* | Total formula weight | 239552.1 |