7OQ4

Cryo-EM structure of the ATV RNAP Inhibitory Protein (RIP) bound to the DNA-binding channel of the host's RNA polymerase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed RNA polymerase subunit A'	polymer	880	99929.1	1	UniProt (P11512) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Sulfolobus acidocaldarius DSM 639
2	B	DNA-directed RNA polymerase subunit B	polymer	1126	126647.1	1	UniProt (P11513) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) In PDB	Sulfolobus acidocaldarius DSM 639
3	C	DNA-directed RNA polymerase subunit A''	polymer	393	44507.4	1	UniProt (P11514) Pfam (PF04998) In PDB	Sulfolobus acidocaldarius DSM 639	Subunit C
4	D	DNA-directed RNA polymerase subunit D	polymer	264	29858.8	1	UniProt (P39471) Pfam (PF01193) Pfam (PF01000) In PDB	Sulfolobus acidocaldarius DSM 639
5	E	DNA-directed RNA polymerase subunit E	polymer	183	20496.8	1	UniProt (P39466) Pfam (PF03876) Pfam (PF00575) In PDB	Sulfolobus acidocaldarius DSM 639
6	F	DNA-directed RNA polymerase, subunit F	polymer	114	13071.0	1	UniProt (Q4JB12) Pfam (PF03874) In PDB	Sulfolobus acidocaldarius DSM 639
7	G	DNA-directed RNA polymerase, subunit G	polymer	130	15292.8	1	UniProt (Q4JAY4) Pfam (PF16992) In PDB	Sulfolobus acidocaldarius DSM 639
8	H	DNA-directed RNA polymerase subunit H	polymer	84	9477.1	1	UniProt (P11521) Pfam (PF01191) In PDB	Sulfolobus acidocaldarius DSM 639
9	K	DNA-directed RNA polymerase subunit K	polymer	89	10252.0	1	UniProt (P39463) Pfam (PF01192) In PDB	Sulfolobus acidocaldarius DSM 639
10	L	DNA-directed RNA polymerase subunit L	polymer	90	10061.8	1	UniProt (P46217) Pfam (PF13656) In PDB	Sulfolobus acidocaldarius DSM 639
11	N	DNA-directed RNA polymerase subunit N	polymer	66	7673.1	1	UniProt (P39472) Pfam (PF01194) In PDB	Sulfolobus acidocaldarius DSM 639
12	P	DNA-directed RNA polymerase subunit P	polymer	48	5662.9	1	UniProt (Q4JAE8) Pfam (PF03604) In PDB	Sulfolobus acidocaldarius DSM 639
13	Q	Conserved protein	polymer	105	12331.7	1	UniProt (Q4JAJ6) Pfam (PF12136) In PDB	Sulfolobus acidocaldarius DSM 639
14	Z	RNAP inhibitory protein	polymer	149	17321.9	1	UniProt (Q3V4R7) Pfam (PF22238) In PDB	Acidianus two-tailed virus (ATV)	RIP
15	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
16	A, B, N, P	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
17	D	FE3-S4 CLUSTER	non-polymer		295.8	1	Chemie (F3S)

Sequence modifications

B: 1 - 1126 (UniProt: P11513)

PDB	External Database	Details
Ile 772	Val 772	variant

G: 1 - 124 (UniProt: Q4JAY4)

PDB	External Database	Details
His 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag

Z: 1 - 145 (UniProt: Q3V4R7)

PDB	External Database	Details
Leu 146	-	expression tag
Val 147	-	expression tag
Pro 148	-	expression tag
Arg 149	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	422583.3
Non-Polymers*	Number of molecules	8
	Total formula weight	712.6
All*	Total formula weight	423295.8

*Water molecules are not included.