7OPP

Crystal structure of the Rab27a fusion with Slp2a-RBDa1 effector for SF4 pocket drug targeting

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	Synaptotagmin-like protein 2,Ras-related protein Rab-27A	polymer	230	25956.2	2	UniProt (Q9HCH5) UniProt (P51159) Pfam (PF02318) Pfam (PF00071)	Homo sapiens (Human)	Breast cancer-associated antigen SGA-72M,Exophilin-4,Rab-27,GTP-binding protein Ram
2	C, E (A, C)	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	2	Chemie (GNP)
3	D, F (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	G, H (A, C)	water	water		18.0	382	Chemie (HOH)

Sequence modifications

A, C: -35 - -8 (UniProt: Q9HCH5)

PDB	External Database	Details
His -37	-	expression tag
Met -36	-	expression tag
Asp -7	-	linker
Gly -6	-	linker
Ser -5	-	linker
Gly -4	-	linker
Ser -3	-	linker
Gly -2	-	linker
Ser -1	-	linker
Gly 0	-	linker

A, C: 1 - 192 (UniProt: P51159)

PDB	External Database	Details
Leu 78	Gln 78	engineered mutation
Ser 123	Cys 123	engineered mutation
Ser 188	Cys 188	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51912.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1093.0
All*	Total formula weight	53005.4

*Water molecules are not included.