7OMM

Cryo-EM structure of N. gonorhoeae LptDE in complex with ProMacrobodies (MBPs have not been built de novo)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	LPS-assembly protein LptD	polymer	801	87656.9	1	UniProt (Q5F651) Pfam (PF04453)	Neisseria gonorrhoeae
2	B (B)	LPS-assembly lipoprotein LptE	polymer	165	18499.1	1	UniProt (A0A5K1Q6A7) Pfam (PF04390) UniProt (by SIFTS) (Q5F9V6)	Neisseria gonorrhoeae
3	C (C)	ProMacrobody 21,Maltodextrin-binding protein	polymer	520	56714.5	1	UniProt (A0A0F8L1I7) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9)	synthetic construct
4	D (D)	ProMacrobody 51,Maltodextrin-binding protein	polymer	526	56861.6	1	UniProt (A0A0F8L1I7) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9)	synthetic construct

Sequence modifications

A: 1 - 801 (UniProt: Q5F651)

PDB	External Database	Details
Ser 13	Ala 13	conflict

B: 1 - 159 (UniProt: A0A5K1Q6A7)

PDB	External Database	Details
His 160	-	expression tag
His 161	-	expression tag
His 162	-	expression tag
His 163	-	expression tag
His 164	-	expression tag
His 165	-	expression tag

C: 1 - 123 (PDB: 7OMM)
C: 124 - 483 (UniProt: A0A0F8L1I7)

PDB	External Database	Details
Pro 484	-	expression tag
Gly 485	-	expression tag
Ser 486	-	expression tag
Gly 487	-	expression tag
Gly 488	-	expression tag
Gly 489	-	expression tag
Ser 490	-	expression tag
Ala 491	-	expression tag
Trp 492	-	expression tag
Ser 493	-	expression tag
His 494	-	expression tag
Pro 495	-	expression tag
Gln 496	-	expression tag
Phe 497	-	expression tag
Glu 498	-	expression tag
Lys 499	-	expression tag
Gly 500	-	expression tag
Gly 501	-	expression tag
Gly 502	-	expression tag
Ser 503	-	expression tag
Gly 504	-	expression tag
Gly 505	-	expression tag
Gly 506	-	expression tag
Ser 507	-	expression tag
Gly 508	-	expression tag
Gly 509	-	expression tag
Ser 510	-	expression tag
Ala 511	-	expression tag
Trp 512	-	expression tag
Ser 513	-	expression tag
His 514	-	expression tag
Pro 515	-	expression tag
Gln 516	-	expression tag
Phe 517	-	expression tag
Glu 518	-	expression tag
Lys 519	-	expression tag
Ala 520	-	expression tag

D: 1 - 129 (PDB: 7OMM)
D: 130 - 489 (UniProt: A0A0F8L1I7)

PDB	External Database	Details
Pro 490	-	expression tag
Gly 491	-	expression tag
Ser 492	-	expression tag
Gly 493	-	expression tag
Gly 494	-	expression tag
Gly 495	-	expression tag
Ser 496	-	expression tag
Ala 497	-	expression tag
Trp 498	-	expression tag
Ser 499	-	expression tag
His 500	-	expression tag
Pro 501	-	expression tag
Gln 502	-	expression tag
Phe 503	-	expression tag
Glu 504	-	expression tag
Lys 505	-	expression tag
Gly 506	-	expression tag
Gly 507	-	expression tag
Gly 508	-	expression tag
Ser 509	-	expression tag
Gly 510	-	expression tag
Gly 511	-	expression tag
Gly 512	-	expression tag
Ser 513	-	expression tag
Gly 514	-	expression tag
Gly 515	-	expression tag
Ser 516	-	expression tag
Ala 517	-	expression tag
Trp 518	-	expression tag
Ser 519	-	expression tag
His 520	-	expression tag
Pro 521	-	expression tag
Gln 522	-	expression tag
Phe 523	-	expression tag
Glu 524	-	expression tag
Lys 525	-	expression tag
Ala 526	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	219732.0
All*	Total formula weight	219732.0

*Water molecules are not included.