7OMG

Crystal structure of KOD DNA Polymerase in a ternary complex with an Uracil containing template

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase	polymer	774	90060.5	1	UniProt (P77933) Pfam (PF03104) Pfam (PF00136) In PDB	Thermococcus kodakarensis KOD1
2	T	Template	polymer	21	6431.1	1		synthetic construct
3	P	Primer	polymer	12	3601.4	1		synthetic construct
4	A	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	1	Chemie (DTP)
5	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	A	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
7	A	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
8	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
9	T	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
10		water	water		18.0	88	Chemie (HOH)

Sequence modifications

A: 1 - 406 (UniProt: P77933)

PDB	External Database	Details
Ala 141	Asp 141	engineered mutation
Ala 143	Glu 143	engineered mutation

A: 407 - 490 (UniProt: P77933)
A: 491 - 774 (UniProt: P77933)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	100093.0
Non-Polymers*	Number of molecules	9
	Total formula weight	834.5
All*	Total formula weight	100927.4

*Water molecules are not included.