7OMB
Crystal structure of KOD DNA Polymerase in a ternary complex with a p/t duplex containing an extended 5' single stranded template overhang
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase | polymer | 774 | 90060.5 | 1 | UniProt (P77933) Pfam (PF03104) Pfam (PF00136) | Thermococcus kodakarensis KOD1 | |
| 2 | B (T) | Template | polymer | 21 | 6470.2 | 1 | synthetic construct | ||
| 3 | C (P) | Primer | polymer | 12 | 3601.4 | 1 | synthetic construct | ||
| 4 | D (A) | 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE | non-polymer | 491.2 | 1 | Chemie (DTP) | |||
| 5 | E, F (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | G, H, I, O (A, P) | MANGANESE (II) ION | non-polymer | 54.9 | 4 | Chemie (MN) | |||
| 7 | J, K, L, M, N (A, T) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 5 | Chemie (EDO) | |||
| 8 | P, Q, R (A, T, P) | water | water | 18.0 | 277 | Chemie (HOH) |
Sequence modifications
A: 1 - 406 (UniProt: P77933)
A: 407 - 490 (UniProt: P77933)
A: 491 - 774 (UniProt: P77933)
| PDB | External Database | Details |
|---|---|---|
| Ala 141 | Asp 141 | engineered mutation |
| Ala 143 | Glu 143 | engineered mutation |
A: 491 - 774 (UniProt: P77933)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 100132.0 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 1069.9 | |
| All* | Total formula weight | 101201.9 |
