7OM7

Thosea asigna virus RdRP domain in complex with RNA and nucleotide UMPNPP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	RNA-dependent RNA polymerase	polymer	684	77110.6	4	UniProt (Q6A562) In PDB	Thosea asigna virus
2	E, F, G, H, I...	RNA (5'-R(P*AP*AP*AP*UP*UP*U)-3')	polymer	8	2495.5	8		synthetic construct
3	A, B	5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine	non-polymer		483.2	2	Chemie (2KH)
4	A	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
5	C	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
6		water	water		18.0	524	Chemie (HOH)

Sequence modifications

A, B, C, D: 11 - 671 (UniProt: Q6A562)

PDB	External Database	Details
Met -12	-	initiating methionine
Gly -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Ser -2	-	expression tag
Gln -1	-	expression tag
Asp 0	-	expression tag
Leu 1	-	expression tag
Glu 2	-	expression tag
Asn 3	-	expression tag
Leu 4	-	expression tag
Tyr 5	-	expression tag
Phe 6	-	expression tag
Gln 7	-	expression tag
Gly 8	-	expression tag
Gly 9	-	expression tag
Ser 10	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	328406.8
Non-Polymers*	Number of molecules	4
	Total formula weight	1127.4
All*	Total formula weight	329534.2

*Water molecules are not included.