7OIN

Crystal structure of LSSmScarlet - a genetically encoded red fluorescent protein with a large Stokes shift

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	LSSmScarlet - Genetically Encoded Red Fluorescent Proteins with a Large Stokes Shift	polymer	274	31091.9	1	UniProt (Q9U6Y8) Pfam (PF01353) In PDB	Discosoma sp.
2	B	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
3	B	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4		water	water		18.0	272	Chemie (HOH)

Sequence modifications

B: 8 - 223 (UniProt: Q9U6Y8)

PDB	External Database	Details
Met -41	-	initiating methionine
Gly -40	-	expression tag
Gly -39	-	expression tag
Ser -38	-	expression tag
His -37	-	expression tag
His -36	-	expression tag
His -35	-	expression tag
His -34	-	expression tag
His -33	-	expression tag
His -32	-	expression tag
Gly -31	-	expression tag
Met -30	-	expression tag
Ala -29	-	expression tag
Ser -28	-	expression tag
Met -27	-	expression tag
Thr -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Gln -23	-	expression tag
Gln -22	-	expression tag
Met -21	-	expression tag
Gly -20	-	expression tag
Arg -19	-	expression tag
Asp -18	-	expression tag
Leu -17	-	expression tag
Tyr -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Lys -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Arg -1	-	expression tag
Ser 0	-	expression tag
Met 1	-	expression tag
Val 2	-	expression tag
Ser 3	-	expression tag
Lys 4	-	expression tag
Gly 5	-	expression tag
Glu 6	-	expression tag
Ala 7	-	expression tag
His 18	Arg 17	engineered mutation
Ser 22	Thr 21	engineered mutation
Met 23	Val 22	engineered mutation
Thr 42	His 41	engineered mutation
Gln 43	Asn 42	engineered mutation
Ala 45	Val 44	engineered mutation
Ser 58	Ala 57	engineered mutation
Nrq 67	Gln 66	chromophore
Nrq 67	Tyr 67	chromophore
Nrq 67	Gly 68	chromophore
Arg 72	Lys 70	engineered mutation
Ala 73	Val 71	engineered mutation
Phe 74	Tyr 72	engineered mutation
Ile 75	Val 73	engineered mutation
His 85	Lys 83	engineered mutation
Gln 87	Leu 85	engineered mutation
Ala 106	Val 104	engineered mutation
Thr 113	Ser 111	engineered mutation
Glu 116	Gln 114	engineered mutation
Thr 119	Cys 117	engineered mutation
Leu 120	Phe 118	engineered mutation
Glu 123	Lys 121	engineered mutation
Leu 126	Phe 124	engineered mutation
Arg 127	Ile 125	engineered mutation
Thr 129	Val 127	engineered mutation
Pro 133	Ser 131	engineered mutation
Leu 145	Trp 143	engineered mutation
Asp 148	Ser 146	engineered mutation
Glu 155	Arg 153	engineered mutation
Asp 162	Glu 160	engineered mutation
Lys 164	His 162	engineered mutation
Met 165	Lys 163	engineered mutation
Arg 168	Lys 166	engineered mutation
Arg 174	His 172	engineered mutation
Ala 177	Val 175	engineered mutation
Asp 178	Glu 176	engineered mutation
Val 179	Phe 177	engineered mutation
Arg 180	Lys 178	engineered mutation
Thr 181	Ser 179	engineered mutation
Thr 182	Ile 180	engineered mutation
Lys 184	Met 182	engineered mutation
Met 191	Leu 189	engineered mutation
Ala 194	Tyr 192	engineered mutation
Asn 196	Tyr 194	engineered mutation
Arg 199	Ser 197	engineered mutation
Val 212	Ile 210	engineered mutation
Ser 219	Thr 217	engineered mutation
Ser 224	-	expression tag
Thr 225	-	expression tag
Gly 226	-	expression tag
Gly 227	-	expression tag
Met 228	-	expression tag
Asp 229	-	expression tag
Glu 230	-	expression tag
Leu 231	-	expression tag
Tyr 232	-	expression tag
Lys 233	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	31091.9
Non-Polymers*	Number of molecules	3
	Total formula weight	142.0
All*	Total formula weight	31233.9

*Water molecules are not included.