7OIK

Mouse RNF213:UBE2L3 transthiolation intermediate, chemically stabilized

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	E3 ubiquitin-protein ligase RNF213	polymer	5161	586545.5	1	UniProt (E9Q555) Pfam (PF00097) Pfam (PF20173)	Mus musculus (house mouse)	Mysterin,RING finger protein 213,RING-type E3 ubiquitin transferase RNF213
2	B (B)	Ubiquitin-conjugating enzyme E2 L3	polymer	166	19197.9	1	UniProt (P68036) Pfam (PF00179)	Homo sapiens (Human)	E2 ubiquitin-conjugating enzyme L3,L-UBC,UbcH7,Ubiquitin carrier protein L3,Ubiquitin-conjugating enzyme E2-F1,Ubiquitin-protein ligase L3
3	C (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
4	D (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	E, F (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 1 - 5148 (UniProt: E9Q555)

PDB	External Database	Details
-	Val 4264	deletion
-	Arg 4265	deletion
-	Asn 4266	deletion
-	Arg 4267	deletion
Gly 5149	-	expression tag
Gly 5150	-	expression tag
Gly 5151	-	expression tag
His 5152	-	expression tag
His 5153	-	expression tag
His 5154	-	expression tag
His 5155	-	expression tag
His 5156	-	expression tag
His 5157	-	expression tag
His 5158	-	expression tag
His 5159	-	expression tag
His 5160	-	expression tag
His 5161	-	expression tag

B: 1 - 154 (UniProt: P68036)

PDB	External Database	Details
Gly -11	-	expression tag
Trp -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
Pro -7	-	expression tag
Gln -6	-	expression tag
Phe -5	-	expression tag
Glu -4	-	expression tag
Lys -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 17	Cys 17	conflict
Ser 137	Cys 137	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	605743.4
Non-Polymers*	Number of molecules	4
	Total formula weight	662.3
All*	Total formula weight	606405.7

*Water molecules are not included.