7OH7

Cryo-EM structure of Drs2p-Cdc50p in the E1-AMPPCP state with PI4P bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Probable phospholipid-transporting ATPase DRS2,Probable phospholipid-transporting ATPase DRS2	polymer	1465	164175.4	1	UniProt (P39524) Pfam (PF16209) Pfam (PF13246) Pfam (PF16212)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
2	B (C)	Cell division control protein 50	polymer	413	47371.8	1	UniProt (P25656) Pfam (PF03381)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
3	C, D (B, D)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	E (E)	beta-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G31886NL)
5	F (A)	(2R)-1-{[(R)-hydroxy{[(1R,2R,3R,4R,5S,6R)-2,3,5,6-tetrahydroxy-4-(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}-3-(octadecanoyloxy)propan-2-yl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate	non-polymer		967.1	1	Chemie (2Y5)
6	G (A)	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	1	Chemie (ACP)
7	H (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8	I (A)	water	water		18.0	3	Chemie (HOH)

Sequence modifications

A: 1 - 1246 (UniProt: P39524)
A: 1253 - 1361 (UniProt: P39524)

PDB	External Database	Details
Ser 1247	-	linker
Leu 1248	-	linker
Val 1249	-	linker
Pro 1250	-	linker
Arg 1251	-	linker
Gly 1252	-	linker
Gly 1362	-	expression tag
Gly 1363	-	expression tag
Gly 1364	-	expression tag
Gly 1365	-	expression tag
Leu 1366	-	expression tag
Val 1367	-	expression tag
Pro 1368	-	expression tag
Arg 1369	-	expression tag
Gly 1370	-	expression tag
Ser 1371	-	expression tag
Gly 1372	-	expression tag
Gly 1373	-	expression tag
Thr 1374	-	expression tag
Ala 1375	-	expression tag
Ala 1376	-	expression tag
Ala 1377	-	expression tag
Pro 1378	-	expression tag
Gly 1379	-	expression tag
Pro 1380	-	expression tag
Ala 1381	-	expression tag
Pro 1382	-	expression tag
Ala 1383	-	expression tag
Pro 1384	-	expression tag
Ala 1385	-	expression tag
Pro 1386	-	expression tag
Ala 1387	-	expression tag
Ser 1388	-	expression tag
Ala 1389	-	expression tag
Pro 1390	-	expression tag
Ala 1391	-	expression tag
Ala 1392	-	expression tag
Ala 1393	-	expression tag
Ala 1394	-	expression tag
Pro 1395	-	expression tag
Ala 1396	-	expression tag
Gly 1397	-	expression tag
Ala 1398	-	expression tag
Gly 1399	-	expression tag
Thr 1400	-	expression tag
Pro 1401	-	expression tag
Val 1402	-	expression tag
Thr 1403	-	expression tag
Ala 1404	-	expression tag
Pro 1405	-	expression tag
Leu 1406	-	expression tag
Ala 1407	-	expression tag
Gly 1408	-	expression tag
Thr 1409	-	expression tag
Ile 1410	-	expression tag
Trp 1411	-	expression tag
Lys 1412	-	expression tag
Val 1413	-	expression tag
Leu 1414	-	expression tag
Ala 1415	-	expression tag
Ser 1416	-	expression tag
Glu 1417	-	expression tag
Gly 1418	-	expression tag
Gln 1419	-	expression tag
Thr 1420	-	expression tag
Val 1421	-	expression tag
Ala 1422	-	expression tag
Ala 1423	-	expression tag
Gly 1424	-	expression tag
Glu 1425	-	expression tag
Val 1426	-	expression tag
Leu 1427	-	expression tag
Leu 1428	-	expression tag
Ile 1429	-	expression tag
Leu 1430	-	expression tag
Glu 1431	-	expression tag
Ala 1432	-	expression tag
Met 1433	-	expression tag
Lys 1434	-	expression tag
Met 1435	-	expression tag
Glu 1436	-	expression tag
Thr 1437	-	expression tag
Glu 1438	-	expression tag
Ile 1439	-	expression tag
Arg 1440	-	expression tag
Ala 1441	-	expression tag
Ala 1442	-	expression tag
Gln 1443	-	expression tag
Ala 1444	-	expression tag
Gly 1445	-	expression tag
Thr 1446	-	expression tag
Val 1447	-	expression tag
Arg 1448	-	expression tag
Gly 1449	-	expression tag
Ile 1450	-	expression tag
Ala 1451	-	expression tag
Val 1452	-	expression tag
Lys 1453	-	expression tag
Ala 1454	-	expression tag
Gly 1455	-	expression tag
Asp 1456	-	expression tag
Ala 1457	-	expression tag
Val 1458	-	expression tag
Ala 1459	-	expression tag
Val 1460	-	expression tag
Gly 1461	-	expression tag
Asp 1462	-	expression tag
Thr 1463	-	expression tag
Leu 1464	-	expression tag
Met 1465	-	expression tag

C: 1 - 391 (UniProt: P25656)

PDB	External Database	Details
Gly 392	-	expression tag
Gly 393	-	expression tag
Gly 394	-	expression tag
Gly 395	-	expression tag
Leu 396	-	expression tag
Val 397	-	expression tag
Pro 398	-	expression tag
Arg 399	-	expression tag
Gly 400	-	expression tag
Ser 401	-	expression tag
Gly 402	-	expression tag
Gly 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag
His 408	-	expression tag
His 409	-	expression tag
His 410	-	expression tag
His 411	-	expression tag
His 412	-	expression tag
His 413	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	211547.2
Branched	Number of molecules	3
	Total formula weight	1597.5
Non-Polymers*	Number of molecules	3
	Total formula weight	1496.6
All*	Total formula weight	214641.3

*Water molecules are not included.