7OFX

Crystal structure of a GH31 family sulfoquinovosidase mutant D455N from Agrobacterium tumefaciens in complex with sulfoquinovosyl glycerol (SQGro)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, C, D, B)	Alpha-glucosidase yihQ	polymer	672	75231.4	4	UniProt (A0A083ZKV2) Pfam (PF01055) Pfam (PF21365) UniProt (by SIFTS) (A9CEZ0)	Rhizobium radiobacter (Agrobacterium tumefaciens)
2	E, F, G, H (A, C, D, B)	[(2S,3S,4S,5R,6S)-6-[(2R)-2,3-bis(oxidanyl)propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid	non-polymer		318.3	4	Chemie (VCW)
3	I, J, K, L (A, C, D, B)	water	water		18.0	439	Chemie (HOH)

Sequence modifications

A, C, D, B: 1 - 664 (UniProt: A0A083ZKV2)

PDB	External Database	Details
Ala 370	Glu 370	engineered mutation
Ala 371	Glu 371	engineered mutation
Asn 455	Asp 455	engineered mutation
Leu 665	-	expression tag
Glu 666	-	expression tag
His 667	-	expression tag
His 668	-	expression tag
His 669	-	expression tag
His 670	-	expression tag
His 671	-	expression tag
His 672	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	300925.8
Non-Polymers*	Number of molecules	4
	Total formula weight	1273.2
All*	Total formula weight	302198.9

*Water molecules are not included.