7OCU
Mannitol-1-phosphate bound to the phosphatase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase MtlD-N374A from Acinetobacter baumannii
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Mannitol-1-phosphate dehydrogenase/phosphatase MtlD | polymer | 727 | 83505.2 | 2 | UniProt (D0C7J2) Pfam (PF13419) Pfam (PF08125) | Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841 | HAD hydrolase, family IA, variant 3 |
| 2 | C (A) | BETA-MERCAPTOETHANOL | non-polymer | 78.1 | 1 | Chemie (BME) | |||
| 3 | D, F, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 4 | E, J, K (A, B) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 5 | G (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | H (B) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 1 | Chemie (EPE) | |||
| 7 | L (B) | D-Mannitol-1-phosphate | non-polymer | 262.2 | 1 | Chemie (44H) | |||
| 8 | M, N (A, B) | water | water | 18.0 | 75 | Chemie (HOH) |
Sequence modifications
A, B: 3 - 716 (UniProt: D0C7J2)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Val 2 | - | expression tag |
| Ala 374 | Asn 373 | engineered mutation |
| Ala 717 | - | expression tag |
| Ala 718 | - | expression tag |
| Ala 719 | - | expression tag |
| Leu 720 | - | expression tag |
| Glu 721 | - | expression tag |
| His 722 | - | expression tag |
| His 723 | - | expression tag |
| His 724 | - | expression tag |
| His 725 | - | expression tag |
| His 726 | - | expression tag |
| His 727 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 167010.5 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 975.1 | |
| All* | Total formula weight | 167985.6 |
