7OCI
Cryo-EM structure of yeast Ost6p containing oligosaccharyltransferase complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 | polymer | 476 | 54116.5 | 1 | UniProt (P41543) Pfam (PF04597) | Saccharomyces cerevisiae S288C | Oligosaccharyl transferase 64 kDa subunit,Oligosaccharyl transferase subunit OST1,Oligosaccharyl transferase subunit alpha |
| 2 | B (B) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 | polymer | 130 | 14712.5 | 1 | UniProt (P46964) Pfam (PF02109) | Saccharomyces cerevisiae S288C | Oligosaccharyl transferase subunit OST2,Oligosaccharyl transferase 16 kDa subunit,Oligosaccharyl transferase subunit epsilon |
| 3 | C (C) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST6 | polymer | 332 | 37921.3 | 1 | UniProt (Q03723) Pfam (PF04756) | Saccharomyces cerevisiae S288C | Oligosaccharyl transferase subunit OST6,Oligosaccharyl transferase 37 kDa subunit,OTase 37 kDa subunit |
| 4 | D (D) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 | polymer | 36 | 3986.7 | 1 | UniProt (Q99380) Pfam (PF10215) | Saccharomyces cerevisiae S288C | Oligosaccharyl transferase subunit OST4,Oligosaccharyl transferase 4 kDa subunit,OTase 4 kDa subunit |
| 5 | E (E) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 | polymer | 86 | 9525.1 | 1 | UniProt (Q92316) Pfam (PF05251) | Saccharomyces cerevisiae S288C | Oligosaccharyl transferase subunit OST5,Oligosaccharyl transferase 9.5 kDa subunit,Oligosaccharyl transferase subunit zeta |
| 6 | F (F) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 | polymer | 718 | 81604.5 | 1 | UniProt (P39007) Pfam (PF02516) Pfam (PF21436) | Saccharomyces cerevisiae S288C | Oligosaccharyl transferase subunit STT3 |
| 7 | G (G) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 | polymer | 430 | 49444.4 | 1 | UniProt (P33767) Pfam (PF03345) Pfam (PF23358) | Saccharomyces cerevisiae S288C | Oligosaccharyl transferase subunit WBP1,Oligosaccharyl transferase subunit beta |
| 8 | H (H) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 | polymer | 286 | 31682.8 | 1 | UniProt (Q02795) Pfam (PF25147) | Saccharomyces cerevisiae S288C | Oligosaccharyl transferase subunit SWP1,Oligosaccharyl transferase subunit delta |
| 9 | I (I) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST6 - TM1 | polymer | 24 | 2060.5 | 1 | Saccharomyces cerevisiae S288C | ||
| 10 | J (K) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 11 | K (J) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G46836GH) | |||
| 12 | L, N, P, Q, T... (A, B, C, D, F...) | 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine | non-polymer | 744.0 | 6 | Chemie (PEE) | |||
| 13 | M, V, W (A, G) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 3 | Chemie (NAG) | |||
| 14 | O, R (B, D) | Digitonin | non-polymer | 1229.3 | 2 | Chemie (AJP) | |||
| 15 | S (F) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 16 | U (F) | Dolichylphosphate | non-polymer | 847.3 | 1 | Chemie (V8K) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 285054.5 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1659.5 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 8458.0 | |
| All* | Total formula weight | 295172.0 |
