7OAF

conserved hypothetical protein residues 311-335 from Candidatus Magnetomorum sp. HK-1 fused to GCN4 adaptors, mutant beta1/A, crystal form III

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	General control transcription factor GCN4,conserved hypothetical protein residues 311-335 from Candidatus Magnetomorum sp. HK-1 fused to GCN4 adaptors, mutant beta1/A,General control transcription factor GCN4	polymer	86	10065.8	3	UniProt (P03069) UniProt (A0A0N0D484) Pfam (PF07716) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Amino acid biosynthesis regulatory protein,General control protein GCN4,Amino acid biosynthesis regulatory protein,General control protein GCN4
2	A	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
3		water	water		18.0	198	Chemie (HOH)

Sequence modifications

A, B, C: 283 - 310 (UniProt: P03069)

PDB	External Database	Details
Gly 278	-	expression tag
Gly 279	-	expression tag
Gly 280	-	expression tag
Ser 281	-	expression tag
Gly 282	-	expression tag
Ile 286	Leu 253	engineered mutation
Met 288	Asp 255	engineered mutation
Ile 290	Val 257	engineered mutation
Ile 293	Leu 260	engineered mutation
Ile 297	Asn 264	engineered mutation
Ile 300	Leu 267	engineered mutation
Ile 304	Val 271	engineered mutation
Ile 307	Leu 274	engineered mutation

A, B, C: 336 - 363 (UniProt: P03069)

PDB	External Database	Details
Ile 339	Leu 253	engineered mutation
Trp 341	Asp 255	engineered mutation
Ile 343	Val 257	engineered mutation
Ile 346	Leu 260	engineered mutation
Ile 350	Asn 264	engineered mutation
Ile 353	Leu 267	engineered mutation
Ile 357	Val 271	engineered mutation
Ile 360	Leu 274	engineered mutation

A, B, C: 311 - 335 (UniProt: A0A0N0D484)

PDB	External Database	Details
Ala 320	Val 320	engineered mutation
Ala 326	Leu 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	30197.3
Non-Polymers*	Number of molecules	1
	Total formula weight	238.3
All*	Total formula weight	30435.6

*Water molecules are not included.