7O87

Crystal structure of holo-F210W mutant of Hydroxy ketone aldolase (SwHKA) from Sphingomonas wittichii RW1 in complex with hydroxypyruvate

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AAA, BBB	HpcH/HpaI aldolase	polymer	252	26823.6	2	UniProt (A5VH82) In PDB	Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)
2	AAA, BBB	3-HYDROXYPYRUVIC ACID	non-polymer		104.1	2	Chemie (3PY)
3	AAA, BBB	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
4	AAA, BBB	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
5	AAA, BBB	POTASSIUM ION	non-polymer		39.1	3	Chemie (K)
6	AAA, BBB	BROMIDE ION	non-polymer		79.9	2	Chemie (BR)
7		water	water		18.0	321	Chemie (HOH)

Sequence modifications

AAA, BBB: 2 - 251 (UniProt: A5VH82)

PDB	External Database	Details
His 0	-	expression tag
His 1	-	expression tag
Trp 210	Phe 210	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	53647.1
Non-Polymers*	Number of molecules	12
	Total formula weight	770.4
All*	Total formula weight	54417.5

*Water molecules are not included.