7O3T

I-layer structure (TrwF/VirB9NTD, TrwE/VirB10NTD) of the outer membrane core complex from the fully-assembled R388 type IV secretion system determined by cryo-EM.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J, M...	TrwE protein	polymer	395	42443.8	16	UniProt (O50337) Pfam (PF03743) In PDB	Escherichia coli
2	B, E, H, K, N...	TrwF protein	polymer	266	29749.6	16	UniProt (O50336) Pfam (PF03524) In PDB	Escherichia coli

Sequence modifications

A, D, G, J, M, P, S, u, V, Y, b, e, h, k, n, y: 1 - 395 (UniProt: O50337)

PDB	External Database	Details
Asp 335	Asn 335	conflict

B, E, H, K, N, Q, T, v, W, Z, c, f, i, l, o, z: 1 - 266 (UniProt: O50336)

PDB	External Database	Details
Asp 71	Ile 71	conflict
Ser 72	Pro 72	conflict
Glu 73	Lys 73	conflict
Ala 74	Pro 74	conflict
Tyr 75	Met 75	conflict
Ala 76	Pro 76	conflict
Phe 77	Leu 77	conflict
Ala 78	Pro 78	conflict
Arg 79	Gly 79	conflict
Lys 80	Arg 80	conflict
Gly 81	Ala 81	conflict
Arg 82	Gly 82	conflict
His 83	Ile 83	conflict
Ile 84	Phe 84	conflict
Phe 85	Leu 85	conflict
Ile 86	Ser 86	conflict
Lys 87	Ser 87	conflict
Pro 88	Arg 88	conflict
Gln 89	Thr 89	conflict

Contents of the asymmetric unit

Polymers	Number of chains	32
	Total formula weight	1155093.9
All*	Total formula weight	1155093.9

*Water molecules are not included.