7O3O

Structure of haloalkane dehalogenase mutant DhaA80(T148L, G171Q, A172V, C176F) from Rhodococcus rhodochrous with ionic liquid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Haloalkane dehalogenase	polymer	299	34266.1	1	UniProt (P0A3G2) Pfam (PF00561) In PDB	Rhodococcus rhodochrous
2	A	ETHANOLAMINE	non-polymer		61.1	1	Chemie (ETA)
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4		water	water		18.0	362	Chemie (HOH)

Sequence modifications

A: 1 - 293 (UniProt: P0A3G2)

PDB	External Database	Details
Leu 148	Thr 148	engineered mutation
Gln 171	Gly 171	engineered mutation
Val 172	Ala 172	engineered mutation
Phe 176	Cys 176	engineered mutation
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	34266.1
Non-Polymers*	Number of molecules	2
	Total formula weight	96.5
All*	Total formula weight	34362.6

*Water molecules are not included.