7O1L

Structure of Mycobacterium tuberculosis beta-oxidation trifunctional enzyme alpha-H462A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	D	Putative acyltransferase Rv0859	polymer	403	42492.4	1	UniProt (O53871) Pfam (PF00108) Pfam (PF02803) In PDB	Mycobacterium tuberculosis H37Rv
2	A	3-hydroxyacyl-CoA dehydrogenase	polymer	736	77938.7	1	UniProt (O53872) Pfam (PF00378) Pfam (PF02737) Pfam (PF00725) In PDB	Mycobacterium tuberculosis H37Rv
3	D, A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4	D	COENZYME A	non-polymer		767.5	1	Chemie (COA)
5	D, A	SULFATE ION	non-polymer		96.1	9	Chemie (SO4)
6		water	water		18.0	130	Chemie (HOH)

Sequence modifications

A: 1 - 720 (UniProt: O53872)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -4	-	expression tag
His -4	-	expression tag
Ser -4	-	expression tag
Gln -4	-	expression tag
Asp -4	-	expression tag
Pro -4	-	expression tag
Asn -4	-	expression tag
Ser -4	-	expression tag
Ala 462	His 462	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	120431.1
Non-Polymers*	Number of molecules	13
	Total formula weight	1908.4
All*	Total formula weight	122339.5

*Water molecules are not included.