7O1I

Structure of Mycobacterium tuberculosis beta-oxidation trifunctional enzyme alpha-E141A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (D)	Putative acyltransferase Rv0859	polymer	403	42476.4	1	UniProt (O53871) Pfam (PF00108) Pfam (PF02803)	Mycobacterium tuberculosis H37Rv
2	B (A)	3-hydroxyacyl-CoA dehydrogenase	polymer	736	77947.8	1	UniProt (O53872) Pfam (PF00378) Pfam (PF02737) Pfam (PF00725)	Mycobacterium tuberculosis H37Rv
3	C (D)	COENZYME A	non-polymer		767.5	1	Chemie (COA)
4	D, J (D, A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	E, F, G, H, I... (D, A)	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
6	P, Q (D, A)	water	water		18.0	44	Chemie (HOH)

Sequence modifications

A: 1 - 720 (UniProt: O53872)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -5	-	expression tag
His -5	-	expression tag
His -5	-	expression tag
Ser -5	-	expression tag
Gln -5	-	expression tag
Asp -5	-	expression tag
Pro -5	-	expression tag
Asn -5	-	expression tag
Ser -5	-	expression tag
Ala 141	Glu 141	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	120424.1
Non-Polymers*	Number of molecules	13
	Total formula weight	1912.4
All*	Total formula weight	122336.5

*Water molecules are not included.