7O1C

Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome-RF2 complex stalled in response to L-tryptophan

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AA	Ribosomal RNA 16S	polymer	1534	497405.0	1	GenBank (1108560344)	Escherichia coli K-12
2	AB	30S ribosomal protein S2	polymer	241	26781.7	1	UniProt (P0A7V0) Pfam (PF00318) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS2
3	AC	30S ribosomal protein S3	polymer	233	26031.3	1	UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS3
4	AD	30S ribosomal protein S4	polymer	206	23514.2	1	UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS4
5	AE	30S ribosomal protein S5	polymer	167	17629.4	1	UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS5
6	AF	30S ribosomal protein S6	polymer	135	15727.5	1	UniProt (P02358) Pfam (PF01250) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein bS6
7	AG	30S ribosomal protein S7	polymer	179	20055.2	1	UniProt (P02359) Pfam (PF00177) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS7
8	AH	30S ribosomal protein S8	polymer	130	14146.6	1	UniProt (P0A7W7) Pfam (PF00410) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS8
9	AI	30S ribosomal protein S9	polymer	130	14886.3	1	UniProt (P0A7X3) Pfam (PF00380) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS9
10	AJ	30S ribosomal protein S10	polymer	103	11755.6	1	UniProt (P0A7R5) Pfam (PF00338) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS10,Transcription termination/antitermination protein NusE
11	AK	30S ribosomal protein S11	polymer	129	13871.0	1	UniProt (P0A7R9) Pfam (PF00411) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS11
12	AL	30S ribosomal protein S12	polymer	124	13814.2	1	UniProt (P0A7S3) Pfam (PF00164) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS12
13	AM	30S ribosomal protein S13	polymer	118	13128.5	1	UniProt (P0A7S9) Pfam (PF00416) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS13
14	AN	30S ribosomal protein S14	polymer	102	11677.6	1	UniProt (P0AG59) Pfam (PF00253) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS14
15	AO	30S ribosomal protein S15	polymer	89	10290.8	1	UniProt (P0ADZ4) Pfam (PF00312) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS15
16	AP	30S ribosomal protein S16	polymer	82	9207.6	1	UniProt (P0A7T3) Pfam (PF00886) In PDB	Escherichia coli K-12	Small ribosomal subunit protein bS16
17	AQ	30S ribosomal protein S17	polymer	84	9724.5	1	UniProt (P0AG63) Pfam (PF00366) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS17
18	AR	30S ribosomal protein S18	polymer	75	9005.5	1	UniProt (P0A7T7) Pfam (PF01084) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein bS18
19	AS	30S ribosomal protein S19	polymer	92	10455.4	1	UniProt (P0A7U3) Pfam (PF00203) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS19
20	AT	30S ribosomal protein S20	polymer	87	9708.5	1	UniProt (P0A7U7) Pfam (PF01649) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein bS20
21	AU	30S ribosomal protein S21	polymer	71	8524.0	1	UniProt (P68679) Pfam (PF01165) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein bS21
22	BA	Ribosomal RNA 23S	polymer	2897	939607.3	1		Escherichia coli K-12
23	BB	Ribosomal RNA 5S	polymer	120	38790.1	1	GenBank (1845258627)	Escherichia coli K-12
24	BC	50S ribosomal protein L2	polymer	273	29923.6	1	UniProt (P60422) Pfam (PF00181) Pfam (PF03947) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL2
25	BD	50S ribosomal protein L3	polymer	209	22291.6	1	UniProt (P60438) Pfam (PF00297) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL3
26	BE	50S ribosomal protein L4	polymer	201	22121.6	1	UniProt (P60723) Pfam (PF00573) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL4
27	BF	50S ribosomal protein L5	polymer	179	20333.6	1	UniProt (P62399) Pfam (PF00281) Pfam (PF00673) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL5
28	BG	50S ribosomal protein L6	polymer	177	18932.8	1	UniProt (P0AG55) Pfam (PF00347) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL6
29	BH	50S ribosomal protein L9	polymer	149	15789.0	1	UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL9
30	BI	50S ribosomal protein L31	polymer	70	7887.1	1	UniProt (P0A7M9) Pfam (PF01197) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL31-A
31	BJ	50S ribosomal protein L13	polymer	142	16050.6	1	UniProt (P0AA10) Pfam (PF00572) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL13
32	BK	50S ribosomal protein L14	polymer	123	13565.1	1	UniProt (P0ADY3) Pfam (PF00238) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL14
33	BL	50S ribosomal protein L15	polymer	144	15008.5	1	UniProt (P02413) Pfam (PF00828) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL15
34	BM	50S ribosomal protein L16	polymer	136	15327.3	1	UniProt (P0ADY7) Pfam (PF00252) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL16
35	BN	50S ribosomal protein L17	polymer	127	14393.7	1	UniProt (P0AG44) Pfam (PF01196) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL17
36	BO	50S ribosomal protein L18	polymer	117	12794.7	1	UniProt (P0C018) Pfam (PF00861) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL18
37	BP	50S ribosomal protein L19	polymer	115	13159.3	1	UniProt (P0A7K6) Pfam (PF01245) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL19
38	BQ	50S ribosomal protein L20	polymer	118	13528.0	1	UniProt (P0A7L3) Pfam (PF00453) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL20
39	BR	50S ribosomal protein L21	polymer	103	11586.4	1	UniProt (P0AG48) Pfam (PF00829) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL21
40	BS	50S ribosomal protein L22	polymer	110	12253.4	1	UniProt (P61175) Pfam (PF00237) In PDB	Escherichia coli K-12	Large ribosomal subunit protein uL22
41	BT	50S ribosomal protein L23	polymer	100	11222.2	1	UniProt (P0ADZ0) Pfam (PF00276) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL23
42	BU	50S ribosomal protein L24	polymer	104	11339.3	1	UniProt (P60624) Pfam (PF00467) Pfam (PF17136) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL24
43	BV	50S ribosomal protein L25	polymer	94	10713.5	1	UniProt (P68919) Pfam (PF01386) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL25
44	BW	50S ribosomal protein L27	polymer	85	9146.5	1	UniProt (P0A7L8) Pfam (PF01016) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL27
45	BX	50S ribosomal protein L28	polymer	78	9027.6	1	UniProt (P0A7M2) Pfam (PF00830) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL28
46	BY	50S ribosomal protein L29	polymer	63	7286.5	1	UniProt (P0A7M6) Pfam (PF00831) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL29
47	BZ	50S ribosomal protein L30	polymer	59	6554.8	1	UniProt (P0AG51) Pfam (PF00327) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL30
48	B0	50S ribosomal protein L32	polymer	57	6463.4	1	UniProt (P0A7N4) Pfam (PF01783) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL32
49	B1	50S ribosomal protein L33	polymer	55	6388.6	1	UniProt (P0A7N9) Pfam (PF00471) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL33
50	B2	50S ribosomal protein L34	polymer	46	5397.5	1	UniProt (P0A7P5) Pfam (PF00468) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL34
51	B3	50S ribosomal protein L35	polymer	65	7313.0	1	UniProt (P0A7Q1) Pfam (PF01632) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL35,Ribosomal protein A
52	B4	50S ribosomal protein L36	polymer	38	4377.4	1	UniProt (P0A7Q6) Pfam (PF00444) In PDB	Escherichia coli K-12	Large ribosomal subunit protein bL36-A,Ribosomal protein B
53	B5	TnaC-(R23F) - Tryptophanase leader peptide	polymer	17	2063.3	1	Pfam (PF08053) UniProt (by SIFTS) (P0AD89) In PDB	Escherichia coli K-12
54	B7	mRNA	polymer	10	3152.9	1		Escherichia coli K-12
55	B8	P-site tRNA-Pro	polymer	77	24846.7	1	GenBank (1847035720)	Escherichia coli K-12
56	B9	Peptide chain release factor RF2	polymer	365	41300.7	1	UniProt (P07012) Pfam (PF03462) Pfam (PF00472) In PDB	Escherichia coli (strain K12)	RF-2
57	AA, BA, BB, BC, BD...	MAGNESIUM ION	non-polymer		24.3	339	Chemie (MG)
58	AA, AM, BA, BB, BC...	POTASSIUM ION	non-polymer		39.1	147	Chemie (K)
59	BI, B4, AB	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
60	BA	TRYPTOPHAN	non-polymer		204.2	1	Chemie (TRP)
61		water	water		18.0	800	Chemie (HOH)

Sequence modifications

AN: 0 - 101 (UniProt: P0AG59)

PDB	External Database	Details
Ala 35	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	56
	Total formula weight	2207277.4
Non-Polymers*	Number of molecules	490
	Total formula weight	14387.3
All*	Total formula weight	2221664.7

*Water molecules are not included.