7NYX

Cryo-EM structure of the MukBEF-MatP-DNA monomer (closed conformation)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Chromosome partition protein MukB	polymer	1482	170240.2	2	UniProt (A0A0F7LRY2) Pfam (PF04310) Pfam (PF16330) Pfam (PF13558)	Photorhabdus thracensis	Structural maintenance of chromosome-related protein
2	C, D (C, D)	Chromosome partition protein MukF	polymer	440	50193.3	2	UniProt (A0A0F7LMQ4) Pfam (PF03882) Pfam (PF17192) Pfam (PF17193)	Photorhabdus thracensis
3	E, F (E, F)	Chromosome partition protein MukE	polymer	240	27423.8	2	UniProt (A0A0F7LPV6) Pfam (PF04288)	Photorhabdus thracensis
4	G, H (G, H)	Acyl carrier protein	polymer	78	8645.5	2	UniProt (A0A6D2XA84) Pfam (PF00550) UniProt (by SIFTS) (P0A6A8)	Escherichia coli BL21(DE3)	ACP
5	I, J (I, J)	Macrodomain Ter protein	polymer	151	18032.6	2	UniProt (A0A0F7LUV5) Pfam (PF17414) Pfam (PF06303)	Photorhabdus thracensis
6	K (K)	matS2 DNA 80 b, oligo FBA769	polymer	80	24625.7	1		synthetic construct
7	L (L)	matS2 DNA 80 b, oligo FBA770	polymer	80	24715.9	1		synthetic construct
8	M, N (M, N)	DNA 80 b	polymer	30	9216.0	2		synthetic construct
9	O, R (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
10	P, Q (A, B)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
11	S, T (G, H)	4'-PHOSPHOPANTETHEINE	non-polymer		358.3	2	Chemie (PNS)

Sequence modifications

A, B: 1 - 1482 (UniProt: A0A0F7LRY2)

PDB	External Database	Details
Gln 1407	Glu 1407	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	616844.5
Non-Polymers*	Number of molecules	6
	Total formula weight	1779.7
All*	Total formula weight	618624.1

*Water molecules are not included.