7NX9

Crystal structure of the N501Y mutant receptor binding domain of SARS-CoV-2 Spike glycoprotein in complex with COVOX-222 and EY6A Fabs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (H)	EY6A Fab heavy chain	polymer	226	24346.3	1		Homo sapiens
2	B (L)	EY6A Fab light chain	polymer	215	23315.9	1		Homo sapiens
3	C (E)	Spike protein S1	polymer	205	23200.0	1	UniProt (P0DTC2) Pfam (PF09408)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2)
4	D (A)	COVOX-222 Fab heavy chain	polymer	224	23461.2	1		Homo sapiens
5	E (B)	COVOX-222 Fab light chain	polymer	214	23327.9	1		Homo sapiens
6	F, G, L, M, N... (H, E, A, B)	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
7	H, J, K (E)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
8	I (E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
9	O (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
10	R (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
11	V, W, X, Y, Z (H, L, E, A, B)	water	water		18.0	255	Chemie (HOH)

Sequence modifications

E: 333 - 528 (UniProt: P0DTC2)

PDB	External Database	Details
Glu 324	-	expression tag
Thr 325	-	expression tag
Gly 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag
Tyr 501	Asn 501	engineered mutation
Lys 527	Pro 527	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	117651.1
Non-Polymers*	Number of molecules	16
	Total formula weight	1599.7
All*	Total formula weight	119250.8

*Water molecules are not included.