7NWT

Initiated 70S ribosome in complex with 2A protein from encephalomyocarditis virus (EMCV)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	50S ribosomal protein L2	polymer	273	29923.6	1	UniProt (P60422) Pfam (PF00181) Pfam (PF03947)	Escherichia coli	ribosomal protein uL2
2	B (C)	50S ribosomal protein L3	polymer	209	22277.5	1	UniProt (P60438) Pfam (PF00297)	Escherichia coli	ribosomal protein uL3
3	C (D)	50S ribosomal protein L4	polymer	201	22121.6	1	UniProt (P60723) Pfam (PF00573)	Escherichia coli	ribosomal protein uL4
4	D (E)	50S ribosomal protein L5	polymer	179	20333.6	1	UniProt (P62399) Pfam (PF00281) Pfam (PF00673)	Escherichia coli	ribosomal protein uL5
5	E (F)	50S ribosomal protein L6	polymer	177	18932.8	1	UniProt (P0AG55) Pfam (PF00347)	Escherichia coli	ribosomal protein uL6
6	F (G)	50S ribosomal protein L9	polymer	149	15789.0	1	UniProt (P0A7R1) Pfam (PF03948) Pfam (PF01281)	Escherichia coli	ribosomal protein bL9
7	G (H)	50S ribosomal protein L10	polymer	165	17736.6	1	UniProt (A0A073UC57)	Escherichia coli
8	H (I)	50S ribosomal protein L11	polymer	142	14894.4	1	UniProt (P0A7J7) Pfam (PF03946) Pfam (PF00298)	Escherichia coli	ribosomal protein uL11
9	I (J)	50S ribosomal protein L13	polymer	142	16050.6	1	UniProt (P0AA10) Pfam (PF00572)	Escherichia coli	ribosomal protein uL13
10	J (K)	50S ribosomal protein L14	polymer	123	13565.1	1	UniProt (P0ADY3) Pfam (PF00238)	Escherichia coli	ribosomal protein uL14
11	K (L)	50S ribosomal protein L15	polymer	144	15008.5	1	UniProt (P02413) Pfam (PF00828)	Escherichia coli	ribosomal protein uL15
12	L (M)	50S ribosomal protein L16	polymer	136	15312.3	1	UniProt (P0ADY7) Pfam (PF00252)	Escherichia coli	ribosomal protein uL16
13	M (N)	50S ribosomal protein L17	polymer	127	14393.7	1	UniProt (P0AG44) Pfam (PF01196)	Escherichia coli	ribosomal protein bL17
14	N (O)	50S ribosomal protein L18	polymer	117	12794.7	1	UniProt (P0C018) Pfam (PF00861)	Escherichia coli	ribosomal protein uL18
15	O (P)	50S ribosomal protein L19	polymer	115	13159.3	1	UniProt (P0A7K6) Pfam (PF01245)	Escherichia coli	ribosomal protein bL19
16	P (Q)	50S ribosomal protein L20	polymer	118	13528.0	1	UniProt (P0A7L3) Pfam (PF00453)	Escherichia coli	ribosomal protein bL20
17	Q (R)	50S ribosomal protein L21	polymer	103	11586.4	1	UniProt (P0AG48) Pfam (PF00829)	Escherichia coli	ribosomal protein bL21
18	R (S)	50S ribosomal protein L22	polymer	110	12253.4	1	UniProt (P61175) Pfam (PF00237)	Escherichia coli	ribosomal protein uL22
19	S (T)	50S ribosomal protein L23	polymer	100	11222.2	1	UniProt (P0ADZ0) Pfam (PF00276)	Escherichia coli	ribosomal protein uL23
20	T (U)	50S ribosomal protein L24	polymer	104	11339.3	1	UniProt (P60624) Pfam (PF00467) Pfam (PF17136)	Escherichia coli	ribosomal protein uL24
21	U (V)	50S ribosomal protein L25	polymer	94	10713.5	1	UniProt (P68919) Pfam (PF01386)	Escherichia coli	ribosomal protein bL25
22	V (W)	50S ribosomal protein L27	polymer	85	9146.5	1	UniProt (P0A7L8) Pfam (PF01016)	Escherichia coli	ribosomal protein bL27
23	W (X)	50S ribosomal protein L28	polymer	78	9027.6	1	UniProt (P0A7M2) Pfam (PF00830)	Escherichia coli	ribosomal protein bL28
24	X (Y)	50S ribosomal protein L29	polymer	63	7286.5	1	UniProt (P0A7M6) Pfam (PF00831)	Escherichia coli	ribosomal protein uL29
25	Y (Z)	50S ribosomal protein L30	polymer	59	6554.8	1	UniProt (P0AG51) Pfam (PF00327)	Escherichia coli	ribosomal protein uL30
26	Z (1)	23S ribosomal RNA	polymer	2904	941832.6	1		Escherichia coli
27	AA (2)	16S ribosomal RNA	polymer	1534	497406.0	1	GenBank (1758835854)	Escherichia coli
28	BA (3)	5S ribosomal RNA	polymer	120	38790.1	1	GenBank (CP035706.1)	Escherichia coli
29	CA (5)	fMet-NH-tRNA(fMet)	polymer	77	24806.9	1	GenBank (1317722521)	Escherichia coli
30	DA (a)	50S ribosomal protein L31	polymer	70	7887.1	1	UniProt (U9XX47)	Escherichia coli
31	EA (b)	50S ribosomal protein L32	polymer	57	6463.4	1	UniProt (P0A7N4) Pfam (PF01783)	Escherichia coli	ribosomal protein bL32
32	FA (c)	50S ribosomal protein L33	polymer	55	6388.6	1	UniProt (P0A7N9) Pfam (PF00471)	Escherichia coli	ribosomal protein bL33
33	GA (d)	50S ribosomal protein L34	polymer	46	5397.5	1	UniProt (P0A7P5) Pfam (PF00468)	Escherichia coli	ribosomal protein bL34
34	HA (e)	50S ribosomal protein L35	polymer	65	7313.0	1	UniProt (P0A7Q1) Pfam (PF01632)	Escherichia coli	Ribosomal protein A, ribosomal protein bL35
35	IA (f)	50S ribosomal protein L36	polymer	38	4377.4	1	UniProt (P0A7Q6) Pfam (PF00444)	Escherichia coli	Ribosomal protein B, ribosomal protein bL36
36	JA (g)	30S ribosomal protein S2	polymer	241	26781.7	1	UniProt (C3TPN2) Pfam (PF00318)	Escherichia coli
37	KA (h)	30S ribosomal protein S3	polymer	233	26031.3	1	UniProt (C3SQX2) Pfam (PF07650) Pfam (PF00189)	Escherichia coli
38	LA (i)	30S ribosomal protein S4	polymer	206	23514.2	1	UniProt (C3SR62) Pfam (PF00163) Pfam (PF01479)	Escherichia coli
39	MA (j)	30S ribosomal protein S5	polymer	167	17629.4	1	UniProt (C3SR27) Pfam (PF00333) Pfam (PF03719)	Escherichia coli
40	NA (k)	30S ribosomal protein S6	polymer	135	15727.5	1	UniProt (P02358) Pfam (PF01250)	Escherichia coli	Small ribosomal subunit protein bS6
41	OA (l)	30S ribosomal protein S7	polymer	179	20055.2	1	UniProt (P02359) Pfam (PF00177)	Escherichia coli	Small ribosomal subunit protein uS7
42	PA (m)	30S ribosomal protein S8	polymer	130	14146.6	1	UniProt (C3SR12) Pfam (PF00410)	Escherichia coli
43	QA (n)	30S ribosomal protein S9	polymer	130	14886.3	1	UniProt (C3SRY2) Pfam (PF00380)	Escherichia coli
44	RA (o)	30S ribosomal protein S10	polymer	103	11755.6	1	UniProt (C3SQT7) Pfam (PF00338)	Escherichia coli
45	SA (p)	30S ribosomal protein S11	polymer	129	13871.0	1	UniProt (C3SR57) Pfam (PF00411)	Escherichia coli
46	TA (q)	30S ribosomal protein S12	polymer	124	13814.2	1	UniProt (P0A7S3) Pfam (PF00164)	Escherichia coli	Small ribosomal subunit protein uS12
47	UA (r)	30S ribosomal protein S13	polymer	118	13128.5	1	UniProt (C3SR52) Pfam (PF00416)	Escherichia coli
48	VA (s)	30S ribosomal protein S14	polymer	101	11606.6	1	UniProt (C3SR07) Pfam (PF00253)	Escherichia coli
49	WA (t)	30S ribosomal protein S15	polymer	89	10290.8	1	UniProt (C3SSQ7) Pfam (PF00312)	Escherichia coli
50	XA (u)	30S ribosomal protein S16	polymer	82	9207.6	1	UniProt (C3SYP2) Pfam (PF00886)	Escherichia coli
51	YA (v)	30S ribosomal protein S17	polymer	84	9724.5	1	UniProt (C3SQY7) Pfam (PF00366)	Escherichia coli
52	ZA (w)	30S ribosomal protein S18	polymer	75	9005.5	1	UniProt (C3SFP7) Pfam (PF01084)	Escherichia coli
53	AB (x)	30S ribosomal protein S19	polymer	92	10455.4	1	UniProt (C3SQW2) Pfam (PF00203)	Escherichia coli
54	BB (y)	30S ribosomal protein S20	polymer	87	9708.5	1	UniProt (C3TRH7) Pfam (PF01649)	Escherichia coli
55	CB (z)	30S ribosomal protein S21	polymer	71	8524.0	1	UniProt (C3STZ7) Pfam (PF01165)	Escherichia coli
56	DB (XX)	mRNA	polymer	117	37684.4	1		Encephalomyocarditis virus
57	EB, FB, GB (AA, BB, CC)	Protein 2A	polymer	152	17831.2	3	UniProt (P12296)	Mengo encephalomyocarditis virus	P2A,G
58	AC, AD, AE, AF, AG... (1, 2, b, i, BB...)	MAGNESIUM ION	non-polymer		24.3	437	Chemie (MG)
59	ZR (5)	N-FORMYLMETHIONINE	non-polymer		177.2	1	Chemie (FME)
60	AS, CS (a, f)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
61	FS (B)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

5: 0 - 76 (GenBank: 1317722521)

PDB	External Database	Details
4su 8	G 2171703	conflict

AA, BB, CC: 1 - 143 (UniProt: P12296)

PDB	External Database	Details
Gly 144	-	expression tag
Ser 145	-	expression tag
Lys 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag
His 151	-	expression tag
His 152	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	59
	Total formula weight	2286655.9
Non-Polymers*	Number of molecules	440
	Total formula weight	10929.3
All*	Total formula weight	2297585.2

*Water molecules are not included.