7NU2
Crystal Structure of Neisseria gonorrhoeae LeuRS E169G mutant in Complex with the Reaction Intermediate Leu-AMP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Leucine--tRNA ligase | polymer | 877 | 98113.3 | 1 | UniProt (B4RNT1) Pfam (PF09334) Pfam (PF13603) Pfam (PF00133) Pfam (PF08264) | Neisseria gonorrhoeae | Leucyl-tRNA synthetase,LeuRS |
| 2 | B, D (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 3 | C (A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 4 | E (A) | [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (2~{S})-2-azanyl-4-methyl-pentanoate | non-polymer | 460.4 | 1 | Chemie (USB) | |||
| 5 | F (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 6 | G (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 7 | H (A) | water | water | 18.0 | 381 | Chemie (HOH) |
Sequence modifications
A: 1 - 876 (UniProt: B4RNT1)
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Gly 169 | Glu 169 | engineered mutation |
| Asn 454 | Asp 454 | conflict |
| Ile 508 | Met 508 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 98113.3 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1181.4 | |
| All* | Total formula weight | 99294.7 |
