7NM6
Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with di-mannuronic acid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Alginate lyase (PL7) | polymer | 231 | 25452.7 | 1 | UniProt (A0A485PVH1) Pfam (PF08787) | Paradendryphiella salina | |
| 2 | B (B) | beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid | branched | 370.3 | 1 | In PDB GlyTouCan (G06440CO) | |||
| 3 | C (A) | 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 209.2 | 1 | Chemie (BTB) | |||
| 4 | D (A) | water | water | 18.0 | 216 | Chemie (HOH) |
Sequence modifications
A: 23 - 243 (UniProt: A0A485PVH1)
| PDB | External Database | Details |
|---|---|---|
| Glu -1 | - | expression tag |
| Phe 0 | - | expression tag |
| Phe 223 | Tyr 223 | engineered mutation |
| Val 244 | - | expression tag |
| Asp 245 | - | expression tag |
| His 246 | - | expression tag |
| His 247 | - | expression tag |
| His 248 | - | expression tag |
| His 249 | - | expression tag |
| His 250 | - | expression tag |
| His 251 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 25452.7 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 370.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 209.2 | |
| All* | Total formula weight | 26032.2 |
