7NL3

Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alginate lyase (PL7)	polymer	231	25452.7	1	UniProt (A0A485PVH1) Pfam (PF08787) In PDB	Paradendryphiella salina
2	A	2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		209.2	1	Chemie (BTB)
3		water	water		18.0	216	Chemie (HOH)

Sequence modifications

A: 23 - 243 (UniProt: A0A485PVH1)

PDB	External Database	Details
Glu -1	-	expression tag
Phe 0	-	expression tag
Phe 223	Tyr 223	engineered mutation
Val 244	-	expression tag
Asp 245	-	expression tag
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	25452.7
Non-Polymers*	Number of molecules	1
	Total formula weight	209.2
All*	Total formula weight	25662.0

*Water molecules are not included.