7NFZ
Crystal structure of haloalkane dehalogenase LinB57 mutant (H272F) from Sphingobium japonicum UT26
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Haloalkane dehalogenase | polymer | 302 | 33982.5 | 1 | UniProt (D4Z2G1) Pfam (PF00561) | Sphingobium japonicum (strain DSM 16413 / CCM 7287 / MTCC 6362 / UT26 / NBRC 101211 / UT26S) | 1,3,4,6-tetrachloro-1,4-cyclohexadiene halidohydrolase,4-TCDN halidohydrolase |
| 2 | B, C, D, E (A) | 3-CYCLOHEXYL-1-PROPYLSULFONIC ACID | non-polymer | 221.3 | 4 | Chemie (CXS) | |||
| 3 | F, G, H (A) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 4 | I (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 5 | J (A) | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 6 | K (A) | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) | |||
| 7 | L (A) | water | water | 18.0 | 246 | Chemie (HOH) |
Sequence modifications
A: 1 - 296 (UniProt: D4Z2G1)
| PDB | External Database | Details |
|---|---|---|
| Phe 272 | His 272 | engineered mutation |
| His 297 | - | expression tag |
| His 298 | - | expression tag |
| His 299 | - | expression tag |
| His 300 | - | expression tag |
| His 301 | - | expression tag |
| His 302 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 33982.5 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1417.8 | |
| All* | Total formula weight | 35400.3 |
