7NF2

Structure of T. atroviride Fdc variant TaFdcV in complex with prFMN crotonic acid adduct

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ferulic acid decarboxylase 1	polymer	519	57019.8	2	UniProt (G9NLP8) Pfam (PF20695) Pfam (PF01977) Pfam (PF20696) In PDB	Hypocrea atroviridis (strain ATCC 20476 / IMI 206040)	Phenacrylate decarboxylase
2	A, B	ACETATE ION	non-polymer		59.0	6	Chemie (ACT)
3	A, B	prFMN cofactor and crotonic acid adduct	non-polymer		566.5	2	Chemie (JRH)
4	A, B	POTASSIUM ION	non-polymer		39.1	4	Chemie (K)
5	A, B	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
6		water	water		18.0	1055	Chemie (HOH)

Sequence modifications

A, B: 1 - 512 (UniProt: G9NLP8)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Asn 25	Glu 25	engineered mutation
Gly 31	Asn 31	engineered mutation
Ala 305	Gly 305	engineered mutation
Arg 351	Asp 351	engineered mutation
His 377	Lys 377	engineered mutation
Val 402	Pro 402	engineered mutation
Tyr 404	Phe 404	engineered mutation
Met 405	Thr 405	engineered mutation
Ala 429	Thr 429	engineered mutation
Pro 445	Val 445	engineered mutation
Trp 448	Gln 448	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	114039.6
Non-Polymers*	Number of molecules	14
	Total formula weight	1753.6
All*	Total formula weight	115793.2

*Water molecules are not included.