7N6V

Crystal structure of HIV-1 Protease multiple mutants PRS17 with Revertant mutation V48G bound to inhibitor Amprenavir

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Protease	polymer	99	10750.6	2	UniProt (P03367) Pfam (PF00077)	Human immunodeficiency virus type 1 group M subtype B (isolate BRU/LAI) (HIV-1)	PR,Retropepsin
2	C (A)	{3-[(4-AMINO-BENZENESULFONYL)-ISOBUTYL-AMINO]-1-BENZYL-2-HYDROXY-PROPYL}-CARBAMIC ACID TETRAHYDRO-FURAN-3-YL ESTER	non-polymer		505.6	1	Chemie (478)
3	D, E (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	F, G (A, B)	water	water		18.0	85	Chemie (HOH)

Sequence modifications

A, B: 1 - 99 (UniProt: P03367)

PDB	External Database	Details
Lys 7	Gln 507	engineered mutation
Ile 10	Leu 510	engineered mutation
Arg 20	Lys 520	engineered mutation
Asp 35	Glu 535	engineered mutation
Ile 36	Met 536	engineered mutation
Asp 37	Ser 537	engineered mutation
Leu 46	Met 546	engineered mutation
Val 54	Ile 554	engineered mutation
Glu 60	Asp 560	engineered mutation
Val 62	Ile 562	engineered mutation
Pro 63	Leu 563	engineered mutation
Ala 67	Cys 567	engineered mutation
Val 71	Ala 571	engineered mutation
Val 72	Ile 572	engineered mutation
Ile 77	Val 577	engineered mutation
Ser 82	Val 582	engineered mutation
Met 90	Leu 590	engineered mutation
Leu 93	Ile 593	engineered mutation
Ala 95	Cys 595	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	21501.1
Non-Polymers*	Number of molecules	3
	Total formula weight	689.8
All*	Total formula weight	22190.9

*Water molecules are not included.