7N4E
Escherichia coli sigma 70-dependent paused transcription elongation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | 1 | DNA (61-MER) | polymer | 61 | 18933.2 | 1 | synthetic construct | ||
| 2 | 2 | DNA (61-MER) | polymer | 61 | 18586.0 | 1 | synthetic construct | ||
| 3 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (A0A073G207) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) In PDB | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 4 | C | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) In PDB | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 5 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (A0A4S1NBU2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) In PDB | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 6 | E | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) In PDB | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 7 | F | RNA polymerase sigma factor RpoD | polymer | 613 | 70282.1 | 1 | UniProt (Q0P6L9) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P00579) In PDB | Escherichia coli | Sigma-70 |
| 8 | R | 5'-R(*UP*UP*CP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3' | polymer | 12 | 3892.4 | 1 | synthetic construct | ||
| 9 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 10 | D | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
D: 1 - 1407 (UniProt: A0A4S1NBU2)
F: 1 - 613 (UniProt: Q0P6L9)
| PDB | External Database | Details |
|---|---|---|
| Val 1384 | Met 1384 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Cys 541 | Arg 541 | engineered mutation |
| Pro 607 | Leu 607 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 501248.2 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 501403.3 |
