7N3T

TrkA ECD complex with designed miniprotein ligand

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	High affinity nerve growth factor receptor	polymer	359	39318.3	2	UniProt (P04629) Pfam (PF16920)	Homo sapiens (Human)	Neurotrophic tyrosine kinase receptor type 1,TRK1-transforming tyrosine kinase protein,Tropomyosin-related kinase A,Tyrosine kinase receptor,Tyrosine kinase receptor A,Trk-A,gp140trk,p140-TrkA
2	C, D (C, D)	Designed TrkA-binding miniprotein	polymer	81	9570.1	2		Synthetic construct
3	E (E)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
4	F (F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
5	AA, DA, EA, G, GA... (B, A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	14	Chemie (NAG)
6	BA, CA, FA, HA, L... (B, A)	1,2-ETHANEDIOL	non-polymer		62.1	9	Chemie (EDO)
7	IA, JA, KA, LA, MA... (B, A)	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
8	NA, OA, PA, QA (A, B, C, D)	water	water		18.0	260	Chemie (HOH)

Sequence modifications

A, B: 36 - 382 (UniProt: P04629)

PDB	External Database	Details
Ser 35	-	expression tag
Leu 263	Val 263	conflict
Ala 383	-	expression tag
Ala 384	-	expression tag
Ala 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag
His 389	-	expression tag
His 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	97776.8
Branched	Number of molecules	2
	Total formula weight	1010.9
Non-Polymers*	Number of molecules	33
	Total formula weight	4616.2
All*	Total formula weight	103403.9

*Water molecules are not included.