7N2C

Elongating 70S ribosome complex in a fusidic acid-stalled intermediate state of translocation bound to EF-G(GDP) (INT2)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	16	16S rRNA	polymer	1534	497390.9	1	GenBank (1789840096)	Escherichia coli K-12
2	SB	30S ribosomal protein S2	polymer	241	26781.7	1	UniProt (P0A7V0) Pfam (PF00318) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS2
3	SC	30S ribosomal protein S3	polymer	233	26031.3	1	UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS3
4	SD	30S ribosomal protein S4	polymer	206	23514.2	1	UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS4
5	SE	30S ribosomal protein S5	polymer	167	17629.4	1	UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS5
6	SF	30S ribosomal protein S6	polymer	135	15727.5	1	UniProt (P02358) Pfam (PF01250) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein bS6
7	SG	30S ribosomal protein S7	polymer	179	20055.2	1	UniProt (P02359) Pfam (PF00177) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS7
8	SH	30S ribosomal protein S8	polymer	130	14146.6	1	UniProt (P0A7W7) Pfam (PF00410) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS8
9	SI	30S ribosomal protein S9	polymer	130	14886.3	1	UniProt (P0A7X3) Pfam (PF00380) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS9
10	SJ	30S ribosomal protein S10	polymer	103	11755.6	1	UniProt (P0A7R5) Pfam (PF00338) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS10
11	SK	30S ribosomal protein S11	polymer	129	13871.0	1	UniProt (P0A7R9) Pfam (PF00411) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS11
12	SL	30S ribosomal protein S12	polymer	124	13814.2	1	UniProt (A0A4S5B3M5) In PDB	Escherichia coli (strain K12)
13	SM	30S ribosomal protein S13	polymer	118	13128.5	1	UniProt (P0A7S9) Pfam (PF00416) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS13
14	SN	30S ribosomal protein S14	polymer	101	11606.6	1	UniProt (P0AG59) Pfam (PF00253) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS14
15	SO	30S ribosomal protein S15	polymer	89	10290.8	1	UniProt (P0ADZ4) Pfam (PF00312) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS15
16	SP	30S ribosomal protein S16	polymer	82	9207.6	1	UniProt (P0A7T3) Pfam (PF00886) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein bS16
17	SQ	30S ribosomal protein S17	polymer	84	9724.5	1	UniProt (P0AG63) Pfam (PF00366) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein uS17
18	SR	30S ribosomal protein S18	polymer	75	9005.5	1	UniProt (P0A7T7) Pfam (PF01084) In PDB	Escherichia coli (strain K12)
19	SS	30S ribosomal protein S19	polymer	92	10455.4	1	UniProt (P0A7U3) Pfam (PF00203) In PDB	Escherichia coli (strain K12)
20	ST	30S ribosomal protein S20	polymer	87	9708.5	1	UniProt (P0A7U7) Pfam (PF01649) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein bS20
21	SU	30S ribosomal protein S21	polymer	71	8524.0	1	UniProt (P68679) Pfam (PF01165) In PDB	Escherichia coli (strain K12)	Small ribosomal subunit protein bS21
22	mR	Chains: mR	polymer	60	19128.4	1		Escherichia coli K-12
23	23	23S rRNA	polymer	2904	941811.6	1	GenBank (802133627)	Escherichia coli K-12
24	5	5S rRNA	polymer	120	38790.1	1	GenBank (991970073)	Escherichia coli K-12
25	LA	50S ribosomal protein L1	polymer	234	24765.7	1	UniProt (A0A4S5ALA4) In PDB	Escherichia coli (strain K12)
26	LB	50S ribosomal protein L2	polymer	273	29923.6	1	UniProt (P60422) Pfam (PF00181) Pfam (PF03947) In PDB	Escherichia coli (strain K12)
27	LC	50S ribosomal protein L3	polymer	209	22277.5	1	UniProt (P60438) Pfam (PF00297) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL3
28	LD	50S ribosomal protein L4	polymer	201	22121.6	1	UniProt (P60723) Pfam (PF00573) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL4
29	LE	50S ribosomal protein L5	polymer	179	20333.6	1	UniProt (P62399) Pfam (PF00281) Pfam (PF00673) In PDB	Escherichia coli (strain K12)
30	LF	50S ribosomal protein L6	polymer	177	18932.8	1	UniProt (P0AG55) Pfam (PF00347) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL6
31	LG	50S ribosomal protein L7/L12	polymer	121	12309.2	1	UniProt (P0A7K2) Pfam (PF16320) Pfam (PF00542) In PDB	Escherichia coli (strain K12)	L8
32	LI	50S ribosomal protein L9	polymer	149	15789.0	1	UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL9
33	LJ	50S ribosomal protein L10	polymer	165	17736.6	1	UniProt (P0A7J3) Pfam (PF00466) In PDB	Escherichia coli (strain K12)	50S ribosomal protein L8,Large ribosomal subunit protein uL10
34	LK	50S ribosomal protein L11	polymer	142	14894.4	1	UniProt (P0A7J7) Pfam (PF03946) Pfam (PF00298) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL11
35	LM	50S ribosomal protein L13	polymer	142	16050.6	1	UniProt (P0AA10) Pfam (PF00572) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL13
36	LN	50S ribosomal protein L14	polymer	123	13565.1	1	UniProt (P0ADY3) Pfam (PF00238) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL14
37	LO	50S ribosomal protein L15	polymer	144	15008.5	1	UniProt (P02413) Pfam (PF00828) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL15
38	LP	50S ribosomal protein L16	polymer	136	15327.3	1	UniProt (P0ADY7) Pfam (PF00252) In PDB	Escherichia coli (strain K12)
39	LQ	50S ribosomal protein L17	polymer	127	14393.7	1	UniProt (P0AG44) Pfam (PF01196) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL17
40	LR	50S ribosomal protein L18	polymer	117	12794.7	1	UniProt (P0C018) Pfam (PF00861) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL18
41	LS	50S ribosomal protein L19	polymer	115	13159.3	1	UniProt (P0A7K6) Pfam (PF01245) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL19
42	LT	50S ribosomal protein L20	polymer	118	13528.0	1	UniProt (P0A7L3) Pfam (PF00453) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL20
43	LU	50S ribosomal protein L21	polymer	103	11586.4	1	UniProt (P0AG48) Pfam (PF00829) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL21
44	LV	50S ribosomal protein L22	polymer	110	12253.4	1	UniProt (P61175) Pfam (PF00237) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL22
45	LW	50S ribosomal protein L23	polymer	100	11222.2	1	UniProt (P0ADZ0) Pfam (PF00276) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL23
46	LX	50S ribosomal protein L24	polymer	104	11339.3	1	UniProt (P60624) Pfam (PF00467) Pfam (PF17136) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL24
47	LY	50S ribosomal protein L25	polymer	94	10713.5	1	UniProt (P68919) Pfam (PF01386) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL25
48	La	50S ribosomal protein L27	polymer	85	9146.5	1	UniProt (P0A7L8) Pfam (PF01016) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL27
49	Lb	50S ribosomal protein L28	polymer	78	9027.6	1	UniProt (P0A7M2) Pfam (PF00830) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL28
50	Lc	50S ribosomal protein L29	polymer	63	7286.5	1	UniProt (P0A7M6) Pfam (PF00831) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL29
51	Ld	50S ribosomal protein L30	polymer	59	6554.8	1	UniProt (P0AG51) Pfam (PF00327) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein uL30
52	Le	50S ribosomal protein L31	polymer	70	7887.1	1	UniProt (P0A7M9) Pfam (PF01197) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL31-A
53	Lf	50S ribosomal protein L32	polymer	57	6463.4	1	UniProt (P0A7N4) Pfam (PF01783) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL32
54	Lg	50S ribosomal protein L33	polymer	55	6388.6	1	UniProt (P0A7N9) Pfam (PF00471) In PDB	Escherichia coli K-12
55	Lh	50S ribosomal protein L34	polymer	46	5397.5	1	UniProt (P0A7P5) Pfam (PF00468) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL34
56	Li	50S ribosomal protein L35	polymer	65	7313.0	1	UniProt (P0A7Q1) Pfam (PF01632) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL35,Ribosomal protein A
57	Lj	50S ribosomal protein L36	polymer	38	4377.4	1	UniProt (P0A7Q6) Pfam (PF00444) In PDB	Escherichia coli (strain K12)	Large ribosomal subunit protein bL36-A,Ribosomal protein B
58	EF	Elongation factor G	polymer	704	77632.1	1	UniProt (P0A6M8) Pfam (PF00009) Pfam (PF03144) Pfam (PF14492) Pfam (PF03764) Pfam (PF00679) In PDB	Escherichia coli (strain K12)	EF-G
59	Pp	Polypeptide	polymer	3	425.6	1		Escherichia coli K-12
60	Pt	Chains: Pt	polymer	76	24703.8	1		Escherichia coli
61	Dt	tRNA	polymer	76	24771.0	1		Escherichia coli
62	23, 16, LC	1,4-DIAMINOBUTANE	non-polymer		88.2	21	Chemie (PUT)
63	16, SN, 23, EF, 5...	MAGNESIUM ION	non-polymer		24.3	197	Chemie (MG)
64	Le, SB, Lj	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
65	23	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
66	23	SPERMIDINE	non-polymer		145.2	3	Chemie (SPD)
67	EF	FUSIDIC ACID	non-polymer		516.7	1	Chemie (FUA)
68	EF	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)

Sequence modifications

EF: 1 - 704 (UniProt: P0A6M8)

PDB	External Database	Details
Ser 1	Met 1	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	61
	Total formula weight	2354385.6
Non-Polymers*	Number of molecules	228
	Total formula weight	9245.5
All*	Total formula weight	2363631.1

*Water molecules are not included.