7MYH

Ubiquitin variant UbV.k.2 in complex with Ube2k

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin-conjugating enzyme E2 K	polymer	201	22501.7	1	UniProt (P61086) Pfam (PF00179) Pfam (PF00627)	Homo sapiens (Human)	E2 ubiquitin-conjugating enzyme K,Huntingtin-interacting protein 2,HIP-2,Ubiquitin carrier protein,Ubiquitin-conjugating enzyme E2-25 kDa,Ubiquitin-conjugating enzyme E2(25K),Ubiquitin-conjugating enzyme E2-25K,Ubiquitin-protein ligase
2	B (B)	Ubiquitin variant UbV.k.2	polymer	95	10461.8	1	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (Human)
3	C (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	D, E (A, B)	water	water		18.0	24	Chemie (HOH)

Sequence modifications

A: 2 - 200 (UniProt: P61086)

PDB	External Database	Details
Met 0	-	expression tag
Ala 1	-	expression tag

B: 1 - 75 (UniProt: P0CG47)

PDB	External Database	Details
Gly -11	-	expression tag
Pro -10	-	expression tag
Gly -9	-	expression tag
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Ser 6	Lys 6	engineered mutation
Phe 8	Leu 8	engineered mutation
Val 9	Thr 9	engineered mutation
Leu 11	Lys 11	engineered mutation
Met 14	Thr 14	engineered mutation
Asn 63	Lys 63	engineered mutation
Asp 64	Glu 64	engineered mutation
Ile 66	Thr 66	engineered mutation
Arg 68	His 68	engineered mutation
Ile 71	Leu 71	engineered mutation
Leu 76	-	expression tag
Leu 77	-	expression tag
Gly 78	-	expression tag
Gly 79	-	expression tag
Gly 80	-	expression tag
Ser 81	-	expression tag
Gly 82	-	expression tag
Gly 83	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	32963.5
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	33055.6

*Water molecules are not included.