7MXG

PRMT5(M420T mutant):MEP50 complexed with inhibitor PF-06855800

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Protein arginine N-methyltransferase 5	polymer	637	72736.6	2	UniProt (O14744) Pfam (PF17285) Pfam (PF05185) Pfam (PF17286)	Homo sapiens (Human)	PRMT5,72 kDa ICln-binding protein,Histone-arginine N-methyltransferase PRMT5,Jak-binding protein 1,Shk1 kinase-binding protein 1 homolog,SKB1 homolog,SKB1Hs
2	B, D (B, D)	Methylosome protein 50	polymer	366	39520.1	2	UniProt (Q9BQA1) Pfam (PF00400)	Homo sapiens (Human)	MEP-50,Androgen receptor cofactor p44,WD repeat-containing protein 77,p44/Mep50
3	E, F (A, C)	7-[(5R)-5-C-(4-chloro-3-fluorophenyl)-beta-D-ribofuranosyl]-7H-pyrrolo[2,3-d]pyrimidin-4-amine	non-polymer		394.8	2	Chemie (ZR4)
4	G, H, I, J (A, B, C, D)	water	water		18.0	805	Chemie (HOH)

Sequence modifications

A, C: 1 - 637 (UniProt: O14744)

PDB	External Database	Details
Thr 420	Met 420	engineered mutation

B, D: 2 - 342 (UniProt: Q9BQA1)

PDB	External Database	Details
Met -23	-	expression tag
Ala -22	-	expression tag
Ser -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Gly -11	-	expression tag
Ala -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Leu 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	224513.3
Non-Polymers*	Number of molecules	2
	Total formula weight	789.6
All*	Total formula weight	225302.9

*Water molecules are not included.