7MTA

Rhodopsin kinase (GRK1)-S5E/S488E/T489E in complex with rhodopsin and Fab1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	G	Rhodopsin kinase GRK1	polymer	543	61542.0	1	UniProt (P28327) Pfam (PF00615) Pfam (PF00069) In PDB	Bos taurus (Bovine)	RK,G protein-coupled receptor kinase 1
2	H	Fab1 Heavy chain	polymer	234	24999.8	1		Homo sapiens
3	L	Fab1 Light chain	polymer	217	23786.4	1		Homo sapiens
4	R	Rhodopsin	polymer	348	39031.5	1	UniProt (P02699) Pfam (PF10413) Pfam (PF00001) In PDB	Bos taurus (Bovine)
5	G	SANGIVAMYCIN	non-polymer		309.3	1	Chemie (SGV)
6	R	RETINAL	non-polymer		284.4	1	Chemie (RET)

Sequence modifications

G: 1 - 535 (UniProt: P28327)

PDB	External Database	Details
Glu 5	Ser 5	engineered mutation
Glu 488	Ser 488	engineered mutation
Glu 489	Thr 489	engineered mutation
Val 536	-	expression tag
Asp 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	149359.6
Non-Polymers*	Number of molecules	2
	Total formula weight	593.7
All*	Total formula weight	149953.4

*Water molecules are not included.