7MRX

Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/C82A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, C, E)	Ribonuclease	polymer	128	14478.1	3	UniProt (P00648)	Bacillus amyloliquefaciens (Bacillus velezensis)	Barnase,RNase Ba
2	B, D, F (B, D, F)	Barstar	polymer	90	10288.6	3	UniProt (P11540)	Bacillus amyloliquefaciens (Bacillus velezensis)	Ribonuclease inhibitor
3	G, H, I, J, K... (A, B, C, D, E...)	water	water		18.0	361	Chemie (HOH)

Sequence modifications

A, C, E: 1 - 110 (UniProt: P00648)

PDB	External Database	Details
Met -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ser -7	-	expression tag
Gln -6	-	expression tag
Ala -5	-	expression tag
Pro -4	-	expression tag
Ile -3	-	expression tag
Glu -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Cys 43	Ala 90	engineered mutation
Cys 80	Ser 127	engineered mutation

B, D, F: 0 - 89 (UniProt: P11540)

PDB	External Database	Details
Ala 40	Cys 41	engineered mutation
Ala 82	Cys 83	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	74300.0
All*	Total formula weight	74300.0

*Water molecules are not included.