7MRX
Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/C82A
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C, E | Ribonuclease | polymer | 128 | 14478.1 | 3 | UniProt (P00648) Pfam (PF00545) In PDB | Bacillus amyloliquefaciens (Bacillus velezensis) | Barnase,RNase Ba |
2 | B, D, F | Barstar | polymer | 90 | 10288.6 | 3 | UniProt (P11540) Pfam (PF01337) In PDB | Bacillus amyloliquefaciens (Bacillus velezensis) | Ribonuclease inhibitor |
3 | water | water | 18.0 | 361 | Chemie (HOH) |
Sequence modifications
A, C, E: 1 - 110 (UniProt: P00648)
B, D, F: 0 - 89 (UniProt: P11540)
PDB | External Database | Details |
---|---|---|
Met -17 | - | expression tag |
Gly -16 | - | expression tag |
Ser -15 | - | expression tag |
Ser -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
His -9 | - | expression tag |
His -8 | - | expression tag |
Ser -7 | - | expression tag |
Gln -6 | - | expression tag |
Ala -5 | - | expression tag |
Pro -4 | - | expression tag |
Ile -3 | - | expression tag |
Glu -2 | - | expression tag |
Gly -1 | - | expression tag |
Arg 0 | - | expression tag |
Cys 43 | Ala 90 | engineered mutation |
Cys 80 | Ser 127 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ala 40 | Cys 41 | engineered mutation |
Ala 82 | Cys 83 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 74300.0 | |
All* | Total formula weight | 74300.0 |