7MPG
Cryo-EM structure of Prefusion-stabilized RSV F (DS-Cav1) in complex with Fab AM14
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Fusion glycoprotein F0,Envelope glycoprotein | polymer | 496 | 54806.5 | 3 | UniProt (A0A0X8XQD7) UniProt (M1E1E4) Pfam (PF00523) UniProt (by SIFTS) (P03420) | Human respiratory syncytial virus | Fusion glycoprotein F1,Fusion glycoprotein F2 |
| 2 | D, F, H (F, H, D) | AM14 Fab Heavy Chain | polymer | 244 | 26380.4 | 3 | Homo sapiens (human) | ||
| 3 | E, G, I (G, I, E) | AM14 Fab Light Chain | polymer | 235 | 25870.9 | 3 | Homo sapiens (human) | ||
| 4 | J, K, L (A, B, C) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 3 | Chemie (NAG) |
Sequence modifications
A, B, C: 26 - 513 (UniProt: A0A0X8XQD7)
A, B, C: 518 - 546 (UniProt: M1E1E4)
| PDB | External Database | Details |
|---|---|---|
| Thr 132 | Ala 93 | conflict |
| - | Arg 98 | deletion |
| - | Arg 99 | deletion |
| - | Glu 100 | deletion |
| - | Leu 101 | deletion |
| - | Pro 102 | deletion |
| - | Arg 103 | deletion |
| - | Phe 104 | deletion |
| - | Met 105 | deletion |
| - | Asn 106 | deletion |
| - | Tyr 107 | deletion |
| - | Thr 108 | deletion |
| - | Leu 109 | deletion |
| - | Asn 110 | deletion |
| - | Asn 111 | deletion |
| - | Thr 112 | deletion |
| - | Lys 113 | deletion |
| - | Asn 114 | deletion |
| - | Asn 115 | deletion |
| - | Asn 116 | deletion |
| - | Val 117 | deletion |
| - | Thr 118 | deletion |
| - | Leu 119 | deletion |
| - | Ser 120 | deletion |
| - | Lys 121 | deletion |
| - | Lys 122 | deletion |
| - | Arg 123 | deletion |
| - | Lys 124 | deletion |
| - | Arg 125 | deletion |
| - | Arg 126 | deletion |
| Val 152 | Ile 142 | conflict |
| Cys 155 | Ser 145 | engineered mutation |
| Phe 190 | Ser 180 | engineered mutation |
| Leu 207 | Val 197 | engineered mutation |
| Cys 290 | Ser 280 | engineered mutation |
| Ser 514 | - | linker |
| Ala 515 | - | linker |
| Ile 516 | - | linker |
| Gly 517 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 546 | Ser 29 | conflict |
| Leu 547 | - | expression tag |
| Val 548 | - | expression tag |
| Pro 549 | - | expression tag |
| Arg 550 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 321173.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 663.6 | |
| All* | Total formula weight | 321836.9 |
