7MNZ

Crystal Structure of Nup358/RanBP2 Ran-binding domain 4 in complex with Ran-GPPNHP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I... (A, C, E, G, I...)	GTP-binding nuclear protein Ran	polymer	216	24430.0	6	UniProt (P62826) Pfam (PF00071)	Homo sapiens (Human)	Androgen receptor-associated protein 24,GTPase Ran,Ras-like protein TC4,Ras-related nuclear protein
2	B, D, F, H, J... (B, D, F, H, J...)	E3 SUMO-protein ligase RanBP2	polymer	139	16188.4	6	UniProt (P49792) Pfam (PF00638)	Homo sapiens (Human)	358 kDa nucleoporin,Nuclear pore complex protein Nup358,Nucleoporin Nup358,Ran-binding protein 2,RanBP2,p270
3	M, O, Q, S, U... (A, C, E, G, I...)	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	6	Chemie (GNP)
4	N, P, R, T, V... (A, C, E, G, I...)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
5	AA, BA, CA, DA, EA... (C, D, E, F, G...)	water	water		18.0	473	Chemie (HOH)

Sequence modifications

A, C, E, G, I, K: 1 - 215 (UniProt: P62826)

PDB	External Database	Details
Ser 0	-	expression tag

B, D, F, H, J, L: 2911 - 3045 (UniProt: P49792)

PDB	External Database	Details
Gly 2907	-	expression tag
Pro 2908	-	expression tag
Gly 2909	-	expression tag
Ser 2910	-	expression tag
Gln 2962	Trp 2962	engineered mutation
Asn 2963	His 2963	engineered mutation
Tyr 2964	Thr 2964	engineered mutation
Asp 2965	Met 2965	engineered mutation
Asn 2966	Lys 2966	engineered mutation
Lys 2967	Asn 2967	engineered mutation
Gln 2968	Tyr 2968	engineered mutation
Val 2969	Tyr 2969	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	243710.8
Non-Polymers*	Number of molecules	12
	Total formula weight	3279.0
All*	Total formula weight	246989.8

*Water molecules are not included.